Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/47257
Título: Activation of the nitric oxide response in gilthead seabream after experimental infection with Photobacterium damselae subsp. piscicida
Autores/as: Acosta, F. 
Ruiz De Galarreta, C. M.
Ellis, A. E.
Díaz, R.
Gómez, V.
Padilla, D. 
Real, F. 
Palabras clave: Pasteurella-Piscicida
Superoxide-Dismutase
Sparus-Aurata
Cells
Inhibition, et al.
Fecha de publicación: 2004
Editor/a: 1050-4648
Publicación seriada: Fish and Shellfish Immunology 
Resumen: Inoculation of small gilthead seabream (Sparus aurata) (30-75 g body weight) with a sublethal dose of different Photobacterium damselae subsp. piscicida (Pdp) strains (DI-21 and 94/99) induced an increase in serum concentrations of stable nitric oxide (NO) metabolites lasting from 6 h to six days post-infection, with a peak at 24 h. In contrast, no such response was detected in larger fish (150-600 g).Since the virulence of Pdp correlates with the presence of a polysaccharide capsular layer which can be induced by growing the bacteria in medium supplemented with 1% glucose (C + forms), the effect of the presence of an enhanced capsular layer on the NO response in small fish was also evaluated. Although, all bacteria induced a similar rapid (6 h) and sustained (up to six days) NO response, serum concentrations of nitrites and citrulline were significantly increased in fish infected with the PdP strains grown in glucose-supplemented medium. When the NO response of fish infected with the C+ form of Pdp was blocked by prior injection of the inhibitor L-NAME, the LD50 was reduced by over 10-fold and the mean time to death was also markedly reduced.Considering that (i) pasteurellosis only affects gilthead seabream with body weights below 100g; (ii) capsulated Pdp are more resistant to the bactericidal action of NO and peroxynitrites than non-capsulated strains; and (iii) blocking the NO response of the fish results in greater susceptibility to Pdp, it seems reasonable to propose that the sustained NO response reported in this study represents a relevant protective mechanism of juvenile gilthead seabream against pasteurellosis. (C) 2003 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/10553/47257
ISSN: 1050-4648
DOI: 10.1016/j.fsi.2003.09.010
Fuente: Fish and Shellfish Immunology[ISSN 1050-4648],v. 16, p. 581-588
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