Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47257
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dc.contributor.authorAcosta, F.-
dc.contributor.authorRuiz De Galarreta, C. M.-
dc.contributor.authorEllis, A. E.-
dc.contributor.authorDíaz, R.-
dc.contributor.authorGómez, V.-
dc.contributor.authorPadilla, D.-
dc.contributor.authorReal, F.-
dc.date.accessioned2018-11-23T12:05:31Z-
dc.date.available2018-11-23T12:05:31Z-
dc.date.issued2004-
dc.identifier.issn1050-4648-
dc.identifier.urihttp://hdl.handle.net/10553/47257-
dc.description.abstractInoculation of small gilthead seabream (Sparus aurata) (30-75 g body weight) with a sublethal dose of different Photobacterium damselae subsp. piscicida (Pdp) strains (DI-21 and 94/99) induced an increase in serum concentrations of stable nitric oxide (NO) metabolites lasting from 6 h to six days post-infection, with a peak at 24 h. In contrast, no such response was detected in larger fish (150-600 g).Since the virulence of Pdp correlates with the presence of a polysaccharide capsular layer which can be induced by growing the bacteria in medium supplemented with 1% glucose (C + forms), the effect of the presence of an enhanced capsular layer on the NO response in small fish was also evaluated. Although, all bacteria induced a similar rapid (6 h) and sustained (up to six days) NO response, serum concentrations of nitrites and citrulline were significantly increased in fish infected with the PdP strains grown in glucose-supplemented medium. When the NO response of fish infected with the C+ form of Pdp was blocked by prior injection of the inhibitor L-NAME, the LD50 was reduced by over 10-fold and the mean time to death was also markedly reduced.Considering that (i) pasteurellosis only affects gilthead seabream with body weights below 100g; (ii) capsulated Pdp are more resistant to the bactericidal action of NO and peroxynitrites than non-capsulated strains; and (iii) blocking the NO response of the fish results in greater susceptibility to Pdp, it seems reasonable to propose that the sustained NO response reported in this study represents a relevant protective mechanism of juvenile gilthead seabream against pasteurellosis. (C) 2003 Elsevier Ltd. All rights reserved.-
dc.publisher1050-4648-
dc.relation.ispartofFish and Shellfish Immunology-
dc.sourceFish and Shellfish Immunology[ISSN 1050-4648],v. 16, p. 581-588-
dc.subject.otherPasteurella-Piscicida-
dc.subject.otherSuperoxide-Dismutase-
dc.subject.otherSparus-Aurata-
dc.subject.otherCells-
dc.subject.otherInhibition-
dc.subject.otherIron-
dc.subject.otherBiosynthesis-
dc.subject.otherMacrophages-
dc.subject.otherExpression-
dc.subject.otherResistance-
dc.titleActivation of the nitric oxide response in gilthead seabream after experimental infection with Photobacterium damselae subsp. piscicida-
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1016/j.fsi.2003.09.010-
dc.identifier.scopus2442624573-
dc.identifier.isi000221496900003-
dc.contributor.authorscopusid56269311600-
dc.contributor.authorscopusid7003806034-
dc.contributor.authorscopusid7201547148-
dc.contributor.authorscopusid7201925856-
dc.contributor.authorscopusid57197332997-
dc.contributor.authorscopusid7005319108-
dc.contributor.authorscopusid7005088705-
dc.contributor.authorscopusid7005397318-
dc.description.lastpage588-
dc.description.firstpage581-
dc.relation.volume16-
dc.type2Artículoes
dc.contributor.daisngid1422265-
dc.contributor.daisngid1664323-
dc.contributor.daisngid196213-
dc.contributor.daisngid6796274-
dc.contributor.daisngid529601-
dc.contributor.daisngid1039753-
dc.contributor.daisngid29464210-
dc.contributor.wosstandardWOS:Acosta, F-
dc.contributor.wosstandardWOS:de Galarreta, CMR-
dc.contributor.wosstandardWOS:Ellis, AE-
dc.contributor.wosstandardWOS:Diaz, R-
dc.contributor.wosstandardWOS:Gomez, V-
dc.contributor.wosstandardWOS:Padilla, D-
dc.contributor.wosstandardWOS:Real, F-
dc.date.coverdateEnero 2004-
dc.identifier.ulpgces
dc.description.jcr2,149
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR ECOAQUA: Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONE HEALTH 2.-Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONEHEALTH 3: Histología y Patología Veterinaria y Forense (Terrestre y Marina)-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.orcid0000-0002-1098-7529-
crisitem.author.orcid0000-0002-6678-5029-
crisitem.author.orcid0000-0001-5281-0521-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameAcosta Arbelo, Félix Antonio-
crisitem.author.fullNamePadilla Castillo, Daniel Fermín-
crisitem.author.fullNameFernández Rodríguez, Antonio Jesús-
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