Identificador persistente para citar o vincular este elemento:
http://hdl.handle.net/10553/47254
Título: | Influence of vaccination on the nitric oxide response of gilthead seabream following infection with Photobacterium damselae subsp. piscicida | Autores/as: | Acosta, F. Real, F. Ellis, A. E. Tabraue, C Padilla, D. Ruiz De Galarreta, C. M. |
Palabras clave: | Macrophage Activating Factor(S) Trout Oncorhynchus-Mykiss Kidney Leukocytes Lipopolysaccharide Release |
Fecha de publicación: | 2005 | Editor/a: | 1050-4648 | Publicación seriada: | Fish and Shellfish Immunology | Resumen: | The nitric oxide (NO) response of vaccinated and non-vaccinated juvenile gilthead seabream was studied in vivo and the NO response of isolated kidney macrophages of fish was studied in vitro. Fish were vaccinated with formalin-killed Photobacterium damselae subsp. piscicida (Pdp) with or without Freund's incomplete adjuvant (FIA) and control fish received phosphate buffered saline (PBS). Thirty days later, fish were injected with a sublethal dose of Pdp and 3 fish/group were bled at time periods thereafter and serum nitrite and citrulline levels were determined as a measure of the NO response. All infected groups showed an increase in NO metabolites from 6 h to 27 days, with peak levels at 24 h. However, the response in bacterin-vaccinated fish was significantly higher than in the non-vaccinated group and the bacterin plus FIA resulted in a further significant enhancement. Similarly enhanced NO responses were produced in vitro by isolated macrophages obtained from vaccinated compared with non-vaccinated fish 30 days after vaccination following infection, with the response in macrophages from fish vaccinated with the bacterin plus FIA being significantly higher than those from fish vaccinated with the bacterin alone. Thus, vaccination resulted in an enhanced NO response to infection with Pdp in vivo and in vitro. Furthermore, the level of protection of fish to experimental challenge with virulent Pdp correlated with the level of the NO responses in the different groups. (C) 2004 Published by Elsevier Ltd. | URI: | http://hdl.handle.net/10553/47254 | ISSN: | 1050-4648 | DOI: | 10.1016/j.fsi.2004.04.004 | Fuente: | Fish & Shellfish Immunology[ISSN 1050-4648],v. 18 (1), p. 31-38 |
Colección: | Artículos |
Citas SCOPUSTM
14
actualizado el 08-sep-2024
Citas de WEB OF SCIENCETM
Citations
13
actualizado el 08-sep-2024
Visitas
79
actualizado el 24-ago-2024
Google ScholarTM
Verifica
Altmetric
Comparte
Exporta metadatos
Los elementos en ULPGC accedaCRIS están protegidos por derechos de autor con todos los derechos reservados, a menos que se indique lo contrario.