Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47250
Title: Expression of the glycoprotein of viral haemorrhagic septicaemia virus (VHSV) on the surface of the fish cell line RTG-P1 induces type 1 interferon expression in neighbouring cells
Authors: Acosta, F. 
Collet, B.
Lorenzen, N.
Ellis, A. E.
Keywords: Rainbow-Trout
Monoclonal-Antibodies
Dna Vaccination
Egtved Virus
Protective Immunity, et al
Issue Date: 2006
Publisher: 1050-4648
Journal: Fish and Shellfish Immunology 
Abstract: In the present study using a luciferase/Mx promoter reporter system, it was shown that the rainbow trout gonad cell line (RTG-P1), a fibroblastic cell line, produces IFN when transfected with a plasmid encoding the glycoprotein of VHSV but not with plasmid vector alone. Only a small percentage of the cells expressed the G protein on the surface membrane as indicated by immunostaining of transfected cells. When transfection was performed in the presence of monoclonal antibodies (Mab) to the glycoprotein, the production of interferon mRNA transcripts was reduced by over 50%. This indicates that the surface expression of G protein was the major mechanism of interferon induction and that most of the interferon was being expressed by cells neighbouring the transfected cells. Crown Copyright (c) 2006 Published by Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/10553/47250
ISSN: 1050-4648
DOI: 10.1016/j.fsi.2005.12.006
Source: Fish and Shellfish Immunology[ISSN 1050-4648],v. 21, p. 272-278
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