Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47247
Title: Invasion and intracellular survival of Hafnia alvei strains in human epithelial cells
Authors: Padilla, D. 
Acosta, F. 
Bravo, J.
Grasso, V.
Real Valcárcel, Fernando 
Vivas, J.
Keywords: Enteropathogenic Escherichia-Coli
In-Vitro
Acute Gastroenteritis
Bacterial Invasion
Genus Hafnia, et al
Issue Date: 2008
Publisher: 1364-5072
Journal: Journal of Applied Microbiology 
Abstract: Aims: The aim of this study was to investigate the invasion and intracellular survival of different Hafnia alvei strains in HeLa cells.Methods and Results: We performed different experiments on the bacterial invasion of different strains of H. alvei into the HeLa cell line using gentamicin protection assays and immunofluorescence. We also report the time course of cell internalization and the effects of inhibitors on the invasion of H. alvei. Levels of invasion varied depending on the conditions (strain, time and inoculum size) used.Conclusions: This study revealed that H. alvei strains were able to enter and persist in a human epithelial cell line.Significance and Impact of the Study: Our in vitro findings highlight the possibility that some H. alvei strains may exploit nonprofessional phagocytes or nonphagocytic cells to spread in vivo, which may be important for the persistence and establishment of an asymptomatic carrier state.
URI: http://hdl.handle.net/10553/47247
ISSN: 1364-5072
DOI: 10.1111/j.1365-2672.2008.03884.x
Source: Journal of Applied Microbiology[ISSN 1364-5072],v. 105, p. 1614-1622
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

13
checked on Jul 25, 2021

WEB OF SCIENCETM
Citations

12
checked on May 23, 2021

Page view(s)

16
checked on Jul 17, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.