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Title: Characterization of the probiotic strain Vagococcus fluvialis in the protection of European sea bass (Dicentrarchus labrax) against vibriosis by Vibrio anguillarum
Authors: Sorroza, L.
Acosta, F. 
Román, L.
Grasso, V.
Padilla Castillo, Daniel Fermín 
Vega, Judit 
Real Valcárcel, Fernando 
UNESCO Clasification: 310907 Patología
Keywords: Lactic-Acid Bacteria
Fish Pathogens
Farmed Fish
Adhesion, et al
Issue Date: 2012
Publisher: 0378-1135
Journal: Veterinary Microbiology 
Abstract: Aquaculture is one of the main sources of income in many countries worldwide. Intensive farms are often affected by different infectious diseases that can decrease their final production. To control this situation, several antibiotics are frequently used with known environmental consequences. The aim of this study was to analyze different bacterial strains isolated from of gilthead sea bream, sea bass, sole and meagre guts, for use as probiotics in aquaculture. The strains were evaluated in vitro through various mechanisms of selection, such as the production of antagonistic effects against pathogens, production of antibacterial substance, adhesion to the intestinal mucus, competition for nutrients or binding site, and growth in intestinal mucus. A total of 50 bacterial strains were analyzed and only one showed excellent in vitro results for consideration as a candidate to be analyzed in vivo. The strain, identified as Vagococcus fluvialis, showed good protection against Vibrio anguillarum 975-1 in vivo in the experimental challenge, showing a relative percent survival of 42.3% higher than positive control group. Therefore, in conclusion we consider this strain to be a good candidate for use as a future probiotic in aquaculture. (C) 2011 Elsevier B.V. All rights reserved.
ISSN: 0378-1135
DOI: 10.1016/j.vetmic.2011.09.013
Source: Veterinary Microbiology [ISSN 0378-1135], v. 155, p. 369-373
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