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Title: In vitro study of adherence, invasion, and persistence of Streptococcus iniae in fibroblastic-like fish cell line SAF-1
Authors: El Aamri, F.
Real Valcárcel, Fernando 
Acosta, F. 
Acosta, B. 
Valdivia, J.
Ramos-Vivas, J.
Padilla, D. 
Keywords: Damselae Subsp Piscicida
Cultured Marine Fish
Sparus-Aurata L
Epithelial-Cells, et al
Issue Date: 2012
Publisher: 0899-7659
Journal: Journal of Aquatic Animal Health 
Abstract: Streptococcus iniae is a major fish pathogen producing invasive infections that result in economic losses in aquaculture. Gentamicin protection assays were used to investigate the ability of different S. iniae strains to invade and adhere to fibroblastic-like fish cell line SAF-1. All strains tested were detected intracellularly using both techniques, with variable internalization degrees between strains. The experiments carried out at 4 degrees C demonstrated that active cell metabolism is necessary for bacterial internalization. Intracellular bacteria were detected for up to 3 d with a round morphology and were stained with 4',6-diamidino-2-phenylindole (DAPI), indicating that some bacterial cells may remain viable inside SAF-1 cells. Our in vitro findings indicate that S. iniae is capable of adhering, entering, and surviving within fibroblastic cells, which may be important for the persistence and establishment of a carrier state.
ISSN: 0899-7659
DOI: 10.1080/08997659.2012.675928
Source: Journal of Aquatic Animal Health[ISSN 0899-7659],v. 24, p. 165-170
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