Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/47232
DC Field | Value | Language |
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dc.contributor.author | Román Fuentes, Lorena | - |
dc.contributor.author | Real Valcárcel, Fernando | - |
dc.contributor.author | El Aamri, F. | - |
dc.contributor.author | Déniz, S. | - |
dc.contributor.author | Grasso, V. | - |
dc.contributor.author | Acosta, F. | - |
dc.contributor.author | Padilla Castillo, Daniel Fermín | - |
dc.date.accessioned | 2018-11-23T11:53:34Z | - |
dc.date.available | 2018-11-23T11:53:34Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | http://hdl.handle.net/10553/47232 | - |
dc.description.abstract | The European sea bass (Dicentrarchus labrax L.) is one of the most extensively farmed marine fish in the Mediterranean sea. Under the high-density condition, common in aquaculture, the infectious diseases can cause significant economic losses. Probiotics are presented as an alternative to antibiotics for the control of aquaculture diseases. This study used real-time PCR to investigate in vitro the dynamic of expression of immune-related genes in sea bass after incubation with live and inactivated (heat and Uv-light) probiotic Vagoccus fluvialis L-21 at different times (T1, T12, T24, T48). The immune associated genes, interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin 10 (IL-10), TumourTumour necrosis factor- (TNF-), ciclo-oxigenase-2 (COX-2), caspase-3 (Casp-3) and Mx were studied in head-kidney (HK) leucocytes of sea bass after incubation with the probiotic strain. Transcript of proinflammatory cytokines (IL-1, TNF-, COX-2) was highly up-regulated after 1 h of incubation with the probiotic strain V. fluvialis L-21. We found statistically significant difference in pick of expression of TNF-, after 1 h of incubation with Uv-light inactivated probiotic strain. The COX-2 expression was highly up-regulated at all times studied, with the exception of 12 and 24 h post incubation for the Uv-light inactivated bacteria. Transcript of IL-10 and Casp-3 showed the higher statistically significant differences of expression after 48 h post incubation with live bacteria. In the contrast, sea bass HK leucocytes expressed Mx at 12 and 48 h without statistically differences among treatments. Our results suggest that V. fluvialis L-21 is able to stimulate in vitro some immune-related genes associated with the early inflammatory response. Future studies in vivo are necessary to clarify this process in sea bass. | - |
dc.language | eng | - |
dc.publisher | 1050-4648 | - |
dc.relation.ispartof | Fish and Shellfish Immunology | - |
dc.source | Fish and Shellfish Immunology[ISSN 1050-4648],v. 35, p. 1329-1332 | - |
dc.subject | 310801 Bacterias | - |
dc.subject | 2415 Biología molecular | - |
dc.subject.other | Probiotic | - |
dc.subject.other | Vagococcus fluvialis L-21 | - |
dc.subject.other | Cytokine expression | - |
dc.subject.other | Leucocytes | - |
dc.subject.other | Sea bass | - |
dc.title | Cytokine expression in head-kidney leucocytes of European sea bass (Dicentrarchus labrax L.) after incubation with the probiotic Vagococcus fluvialis L-21 | - |
dc.type | info:eu-repo/semantics/Article | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.fsi.2013.07.036 | - |
dc.identifier.pmid | 23927874 | - |
dc.identifier.scopus | 84883557195 | - |
dc.identifier.isi | 000324849800032 | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.authorscopusid | 50661926900 | - |
dc.contributor.authorscopusid | 7005397318 | - |
dc.contributor.authorscopusid | 7005088705 | - |
dc.contributor.authorscopusid | 55351966600 | - |
dc.contributor.authorscopusid | 7003859353 | - |
dc.contributor.authorscopusid | 23060092500 | - |
dc.contributor.authorscopusid | 56269311600 | - |
dc.description.lastpage | 1332 | - |
dc.identifier.issue | 4 | - |
dc.description.firstpage | 1329 | - |
dc.relation.volume | 35 | - |
dc.investigacion | Ciencias de la Salud | - |
dc.type2 | Artículo | - |
dc.contributor.daisngid | 6180345 | - |
dc.contributor.daisngid | 29464210 | - |
dc.contributor.daisngid | 1039753 | - |
dc.contributor.daisngid | 3017971 | - |
dc.contributor.daisngid | 28148410 | - |
dc.contributor.daisngid | 345205 | - |
dc.contributor.daisngid | 878226 | - |
dc.utils.revision | Sí | - |
dc.contributor.wosstandard | WOS:Roman, L | - |
dc.contributor.wosstandard | WOS:Real, F | - |
dc.contributor.wosstandard | WOS:Padilla, D | - |
dc.contributor.wosstandard | WOS:El Aamri, F | - |
dc.contributor.wosstandard | WOS:Deniz, S | - |
dc.contributor.wosstandard | WOS:Grasso, V | - |
dc.contributor.wosstandard | WOS:Acosta, F | - |
dc.date.coverdate | Enero 2013 | - |
dc.identifier.ulpgc | Sí | - |
dc.contributor.buulpgc | BU-VET | - |
dc.description.sjr | 1,001 | - |
dc.description.jcr | 3,034 | - |
dc.description.sjrq | Q1 | - |
dc.description.jcrq | Q1 | - |
dc.description.scie | SCIE | - |
item.fulltext | Sin texto completo | - |
item.grantfulltext | none | - |
crisitem.author.dept | GIR IUSA-ONE HEALTH 2 - Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria | - |
crisitem.author.dept | IU de Sanidad Animal y Seguridad Alimentaria | - |
crisitem.author.dept | Departamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos | - |
crisitem.author.dept | GIR IUSA-ONE HEALTH 2 - Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria | - |
crisitem.author.dept | IU de Sanidad Animal y Seguridad Alimentaria | - |
crisitem.author.dept | Departamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos | - |
crisitem.author.dept | GIR Grupo de Investigación en Acuicultura | - |
crisitem.author.dept | IU de Investigación en Acuicultura Sostenible y Ec | - |
crisitem.author.dept | Departamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos | - |
crisitem.author.dept | GIR IUSA-ONE HEALTH 2 - Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria | - |
crisitem.author.dept | IU de Sanidad Animal y Seguridad Alimentaria | - |
crisitem.author.dept | Departamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos | - |
crisitem.author.orcid | 0000-0001-6526-0354 | - |
crisitem.author.orcid | 0000-0003-0201-8338 | - |
crisitem.author.orcid | 0000-0002-1098-7529 | - |
crisitem.author.orcid | 0000-0002-6678-5029 | - |
crisitem.author.parentorg | IU de Sanidad Animal y Seguridad Alimentaria | - |
crisitem.author.parentorg | IU de Sanidad Animal y Seguridad Alimentaria | - |
crisitem.author.parentorg | IU de Investigación en Acuicultura Sostenible y Ec | - |
crisitem.author.parentorg | IU de Sanidad Animal y Seguridad Alimentaria | - |
crisitem.author.fullName | Real Valcárcel, Fernando | - |
crisitem.author.fullName | Déniz Suárez, María Soraya | - |
crisitem.author.fullName | Acosta Arbelo, Félix Antonio | - |
crisitem.author.fullName | Padilla Castillo, Daniel Fermín | - |
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