Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47230
Title: Experimental Lactococcus garvieae infection in zebrafish and first evidence of its ability to invade non-phagocytic cells
Authors: Aguado-Urda, Mónica
Rodríguez-Bertos, Antonio
de las Heras, Ana I.
Blanco, María M.
Acosta, Félix 
Cid, Raquel
Fernández-Garayzábal, José F.
Gibello, Alicia
Keywords: Streptococcus-Iniae
In-Vitro
Fish
Pathogenicity
Yellowtail, et al
Issue Date: 2014
Publisher: 0378-1135
Journal: Veterinary Microbiology 
Abstract: Zebrafish has been used for studying infections and host pathogen interactions in different bacterial fish pathogens. In the present study we evaluated the ability of Lactococcus garvieae to infect zebrafish when inoculated intraperitoneally with 2 x 10(7) UFC of this pathogen. L. garvieae can colonize and invade zebrafish at multiple anatomical sites causing a lethal acute septicemic infection with clinical signs and lesions consistent with those observed in lactococcosis outbreaks. Immunohistochemical studies showed the presence of L garvieae into macrophages as well as into non-phagocytic zebrafish cells of liver (hepatocytes). The internalization capacity, showed by L garvieae in zebrafish cells was confirmed in the rainbow trout cell line RTG-2. Our results provide the first evidence that L garvieae is able to invade non-phagocytic host cells. (C) 2014 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/10553/47230
ISSN: 0378-1135
DOI: 10.1016/j.vetmic.2014.03.029
Source: Veterinary Microbiology[ISSN 0378-1135],v. 171, p. 248-254
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

10
checked on Dec 4, 2022

WEB OF SCIENCETM
Citations

8
checked on May 23, 2021

Page view(s)

14
checked on Sep 10, 2022

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.