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Title: Interactions of Streptococcus iniae with phagocytic cell line
Authors: El Aamri, Fatima
Remuzgo-Martínez, S.
Acosta, Félix 
Real Valcárcel, Fernando 
Ramos-Vivas, José
Icardo, José M.
Padilla, Daniel 
UNESCO Clasification: 310905 Microbiología
Keywords: Aquatic Pathogen
Virulence Factor
Membrane Attack
Fish, et al
Issue Date: 2015
Publisher: 1286-4579
Journal: Microbes and Infection 
Abstract: Streptococcus iniae has become one of the most serious aquatic pathogens in the last decade, causing large losses in wild and farmed fish worldwide. There is clear evidence that this pathogen is capable not only of causing serious disease in fish but also of being transferred to and infecting humans. In this study, we investigate the interaction of S. iniae with two murine macrophage cell lines, J774-A1 and RAW 264.7. Cytotoxicity assay demonstrated significant differences between live and UV-light killed IUSA-1 strains. The burst respiratory activity decreased to baseline after 1 and 4 h of exposure for J774-A1 and RAW 264.7, respectively. Immunofluorescent and ultrastructural study of infected cells confirmed the intracellular localization of bacteria at 1 h and 24 h post-infection. Using qRT-PCR arrays, we investigated the changes in the gene expression of immune relevant genes associated with macrophage activation. In this screening, we identified 11 of 84 genes up-regulated, we observed over-expression of pro-inflammatory response as IL-1 alpha, IL-1 beta, and TNF-alpha, without a good anti-inflammatory response. Present findings suggest a capacity of S. iniae to modulate a mammalian macrophages cell lines to their survival and replication intracellular, which makes this cell type as a reservoir for continued infection. (C) 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
ISSN: 1286-4579
DOI: 10.1016/j.micinf.2014.06.006
Source: Microbes and Infection[ISSN 1286-4579],v. 17, p. 258-265
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