Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/47223
Título: Immunization of sea bream (Sparus aurata) juveniles against Photobacterium damselae subsp. piscicida by short bath: Effect on some pro-inflammatory molecules and the Mx gene expression
Autores/as: Grasso, V.
Padilla, D. 
Bravo García, Jimena 
Román, L.
Rosario, I. 
Acosta, B. 
Vega, B.
El Aamri, F.
Escuela, O.
Ramos-Vivas, J.
Acosta, F. 
Palabras clave: Dicentrarchus-Labrax Interleukin-1-Beta
Trout Oncorhynchus-Mykiss
Double-Stranded-Rna
Salmo-Salar L.
Rainbow-Trout, et al.
Fecha de publicación: 2015
Editor/a: 1050-4648
Publicación seriada: Fish and Shellfish Immunology 
Resumen: Cytokines are a family of proteins derived from macrophages, lymphocytes, granulocytes, mast cells and epithelial cells and can be divided into interferons (IFNs), Interleukins (ILs) and Tumor Necrosis factors (TNFs) among others. The presence of cytokines in a wide number of fish species has been proved and several molecules types have been already cloned and sequenced. In this work some proinflamatory molecules and Mx gene were detected in the liver of vaccinated sea bream juveniles with an average body weight of 5 g. The method of immunization was by short bath and three different bacterins against the marine pathogen Photobacterium damselae subsp. piscicida were designed and used to immunize fish. Five genes encoding for five different molecules were analyzed by real time PCR: IL-1 beta, IL Ir-2, Cox-2, Mx and TNF alpha. Gene expression was quantified along four days after fish immunization and results were compared among groups. Results show that the heat-inactivated vaccine stimulates the up-regulation of IL-1 beta, IL Ir-2, Cox-2 and TNFa genes whereas the UV-light inactivated vaccine was the unique vaccine which stimulates the expression of Mx gene. The present is a novel study that shows by the first time the effect of the inactivation process of vaccines on the expression levels of genes involved in the defense against Photobacterium damselae subsp piscicida. (C) 2015 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/10553/47223
ISSN: 1050-4648
DOI: 10.1016/j.fsi.2015.06.030
Fuente: Fish and Shellfish Immunology[ISSN 1050-4648],v. 46, p. 292-296
Colección:Artículos
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