Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47182
Title: Determination of free amino acids in microalgae by high-performance liquid chromatography using pre-column fluorescence derivatization
Authors: Betancort Rodríguez, J. R.
Garcia-Blairsy Reina, Guillermo 
Santana Rodríguez, J. J. 
UNESCO Clasification: 2301 química analítica
230103 Análisis cromatográfico
241707 Algología (ficología)
Keywords: Microalgae
HPLC
Issue Date: 1997
Publisher: 0269-3879
Journal: Biomedical Chromatography 
Abstract: An analytical method for a rapid reverse-phase liquid chromatography of amino acids is presented. The totalanalysis time was 19 min. The average relative deviation over the measured peak area was lower than 10% andthe fluorescent response was linear with concentration for OPA derivatives (r > 0.99) in the range 100–1000 nM.The proposed method was applied to the determination of free amino acids in two microalgae species (Chlorellapyrenoidosa and Chlamydomonas rinhardtii). The principal aminoacids found were alanine, arginine leucine,serine and glutamic acid.
URI: http://hdl.handle.net/10553/47182
ISSN: 0269-3879
DOI: 10.1002/(SICI)1099-0801(199711)11:6<335::AID-BMC684>3.3.CO;2-K
Source: Biomedical chromatography [ISSN 1099-0801], v. 11, p. 335-336
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