Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47057
Title: Phenolic compounds, antioxidant activity and ultrastructural study from Protea hybrid 'Susara'
Authors: León, Francisco
Alfayate, Carmen
Batista, Candelaria Vera
López, Aroa
Rico, Milagros 
Brouard, Ignacio
UNESCO Clasification: 230690 Química de productos naturales orgánicos
Keywords: Protea hybrid ‘Susara’
Antioxidant activity
Phenolic compounds
Proteaceae
Issue Date: 2014
Publisher: 0926-6690
Journal: Industrial Crops and Products 
Abstract: In the present study we investigated the chemical composition and the phenolic contents of the ethanolic extract from aerial parts of Protea hybrid ‘Susara’, its antioxidant activity and also the structure and ultrastructure of the leaves and stems by light microscopy (LM) and transmission electron microscopy (TEM). Phytochemical research led to the isolation of twelve compounds including: (6-ethoxy-3,5-dihydroxy-4-oxotetrahydro-2H-pyran-2-yl)methyl 4-hydroxybenzoate (1), 4-ethoxy-2,3-dihydroxy-4-oxobutyl 4-hydroxybenzoate (3), 2-ethoxy-4-(hydroxymethyl)phenol (4) and 1,5-anhydro-d-glucitol (5). Moreover, fourteen phenolic compounds were identified and quantified by RP-HPLC, and the predominant was catechin, followed by gallic and syringic acids. Compound 4 showed the highest radical scavenging activity (98%) with a half-time (t1/2) of 10 s. In TEM, the phenolic compounds were osmiophilic, appearing within the cell lumen of different tissues as isolated droplets of different sizes and fine granular material. Aerial parts presented thick cuticles and heavily lignified tissues with strong reinforcements of sclerenchyma, all indicating scleromorphic features. Our finding suggested that some agriculture waste biomass could be converted into high-added value products with a potential use within the food preservation, pharmaceutical, cosmetic and therapeutic industries.
URI: http://hdl.handle.net/10553/47057
ISSN: 0926-6690
DOI: 10.1016/j.indcrop.2014.02.024
Source: Industrial Crops and Products [ISSN 0926-6690], v. 55, p. 230-237
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