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Title: The 2000-2001 GEP-ISFG Collaborative Exercise on mtDNA: Assessing the cause of unsuccessful mtDNA PCR amplification of hair shaft samples
Authors: Prieto, Lourdes
Montesino, Marta
Salas, Antonio
Alonso, Antonio
Albarrán, Cristina
Álvarez, Sara
Crespillo, Manuel
Di Lonardo, Ana Ma
Doutremepuich, Christian
Fernández-Fernández, Isabel
De La Vega, Alberto González
Gusmão, Leonor
López, Carlos M.
López-Soto, Manolo
Lorente, José A.
Malaghini, Marcelo
Martínez, Carlos A.
Modesti, Nidia M.
Palacio, Ana Ma
Paredes, Manuel
Pena, Sergio D.J.
Pérez-Lezaun, Anna
Pestano, José J. 
Puente, Jorge
Sala, Andrea
Vide, Ma Conceiçao
Whittle, Martín R.
Yunis, Juan J.
Gómez, Josefina
UNESCO Clasification: 32 Ciencias médicas
320102 Genética clínica
3203 Medicina forense
Keywords: mtDNA
Hair shaft samples
Issue Date: 2003
Journal: Forensic Science International 
Abstract: We report the results of Spanish and Portuguese working group (GEP) of International Society of Forensic Genetics (ISFG) Collaborative Exercise 2001-2002 on mitochondrial DNA (mtDNA) analysis. 64 laboratories from Spain, Portugal and several Latin-American countries participated in this quality control exercise. Five samples were sent to the participating laboratories, four blood stains (M1-M4) and a sample (M5) consisting of two hair shaft fragments. M4 was non-human (Felis catus) in origin; therefore, the capacity of the labs to identify the biological source of this sample was an integral part of the exercise. Some labs detected the non-human origin of M4 by carrying out immuno-diffussion techniques using antihuman serum, whereas others identified the specific animal origin by testing the sample against a set of animal antibodies or by means of the analysis of mtDNA regions (Cyt-b, 12S, and 16S genes). The results of the other three human blood stains (M1-M3) improved in relation to the last Collaborative Exercises but those related to hairs yielded a low rate of success which clearly contrasts with previous results. As a consequence of this, some labs performed additional analysis showing that the origin of this low efficiency was not the presence of inhibitors, but the low quantity of DNA present in these specific hair samples and the degradation. As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for the labs performing mtDNA analysis in forensic casework.
ISSN: 0379-0738
DOI: 10.1016/S0379-0738(03)00095-1
Source: Forensic Science International[ISSN 0379-0738],v. 134, p. 46-53
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