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Title: Forensic analysis of dog (Canis lupus familiaris) mitochondrial DNA sequences: An inter-laboratory study of the GEP-ISFG working group
Authors: van Asch, Barbara
Albarran, Cristina
Alonso, Antonio
Angulo, Ramón
Alves, Cíntia
Betancor, Eva 
Catanesi, Cecilia I.
Corach, Daniel
Crespillo, Manuel
Doutremepuich, Christian
Estonba, Andone
Fernandes, Ana Teresa
Fernandez, Eugenia
Garcia, Ana Maria
Garcia, Miguel Angel
Gilardi, Patricia
Gonçalves, Rita
Hernandez, A
Lima, Gabriela
Nascimento, Eugênio
de Pancorbo, Marian M.
Parra, David
Pinheiro, Maria de Fátima
Prat, Elena
Puente, Jorge
Ramírez, José Luis
Rendo, Fernando
Rey, Isabel
Di Rocco, Florencia
Rodriguez, Anayanci
Sala, Andrea
Salla, Joel
Sanchez, Juan J.
Solá, Dácil
Silva, Sandra
Pestano Brito, José Juan 
Amorim, António
UNESCO Clasification: 240108 Genética animal
Keywords: Proficiency Testing Program
Control Region
Domestic Dog
Gata-A10, et al
Issue Date: 2009
Journal: Forensic Science International: Genetics 
Abstract: A voluntary collaborative exercise aiming at the mitochondrial analysis of canine biological samples was carried out in 2006-2008 by the Non-Human Forensic Genetics Commission of the Spanish and Portuguese WorkingGroup(GEP) of the International Society for Forensic Genetics (ISFG). The participating laboratories were asked to sequence two dog samples (one blood stain and one hair sample) for the mitochondrial D-loop region comprised between positions 15,372 and 16,083 using suggested primers and PCR conditions, and to compare their results against a reference sequence. Twenty-one participating laboratories reported a total of 67.5% concordant results, 15% non-concordant results, and 17.5% no results. The hair sample analysis presented more difficulty to the participants than the bloodstain analysis, with a high percentage (29%) failing to obtain a result. The high level of participation showed the interest of the community in the analysis of dog forensic samples but the results reveal that crucial methodological issues need to be addressed and further training is required in order to respond proficiently to the demands of forensic casework.
ISSN: 1872-4973
DOI: 10.1016/j.fsigen.2009.04.008
Source: Forensic Science International-Genetics [ISSN 1872-4973], v. 4 (1), p. 49-54
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