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http://hdl.handle.net/10553/46483
Título: | Polyamines in marine macroalgae: Levels of putrescine, spermidine and spermine in the thalli and changes in their concentration during glycerol-induced cell growth in vitro | Autores/as: | Marián, F. D. García-Jiménez, P. Robaina, R. R. |
Clasificación UNESCO: | 241719 Fisiología vegetal 251004 Botánica marina |
Palabras clave: | Plant Development Porphyridium Sp Morphogenesis |
Fecha de publicación: | 2000 | Editor/a: | 0031-9317 | Publicación seriada: | Physiologia Plantarum | Resumen: | The levels of putrescine (Put), spermidine (Spd) and spermine (Spm) were analyzed in naturally collected samples of the marine macroalgae Dyctiota dichotoma, Gelidium canariensis and Grateloupia doryphora. Polyamines (PAs) appeared in free (35–134 μg g−1 fresh weight) and bound TCA‐insoluble form (1 667–2 624 μg g−1 fresh weight). Axenic in vitro cultures of sporelings from G. doryphora were established in the medium containing glycerol. This medium promoted growth and morphogenesis and also increased the free and bound PA levels in the sporelings. Tracer experiments using 70 kBq [U‐14C]‐glycerol showed significant quantities of radioactivity in Put, Spd and Spm after 20 h of incubation. The effects of glycerol on growth were inhibited by the ornithine decarboxylase (EC 4.1.1.17) inhibitor α‐difluoromethylornithine (DFMO). The presence of DFMO in the incubation medium with [U‐14C]‐glycerol also reduced the radioactivity in PAs. | URI: | http://hdl.handle.net/10553/46483 | ISSN: | 0031-9317 | DOI: | 10.1034/j.1399-3054.2000.1100416.x | Fuente: | Physiologia Plantarum [ISSN 0031-9317], v. 110 (4), p. 530-534 |
Colección: | Artículos |
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