Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/46483
Título: Polyamines in marine macroalgae: Levels of putrescine, spermidine and spermine in the thalli and changes in their concentration during glycerol-induced cell growth in vitro
Autores/as: Marián, F. D.
García-Jiménez, P. 
Robaina, R. R. 
Clasificación UNESCO: 241719 Fisiología vegetal
251004 Botánica marina
Palabras clave: Plant Development
Porphyridium Sp
Morphogenesis
Fecha de publicación: 2000
Editor/a: 0031-9317
Publicación seriada: Physiologia Plantarum 
Resumen: The levels of putrescine (Put), spermidine (Spd) and spermine (Spm) were analyzed in naturally collected samples of the marine macroalgae Dyctiota dichotoma, Gelidium canariensis and Grateloupia doryphora. Polyamines (PAs) appeared in free (35–134 μg g−1 fresh weight) and bound TCA‐insoluble form (1 667–2 624 μg g−1 fresh weight). Axenic in vitro cultures of sporelings from G. doryphora were established in the medium containing glycerol. This medium promoted growth and morphogenesis and also increased the free and bound PA levels in the sporelings. Tracer experiments using 70 kBq [U‐14C]‐glycerol showed significant quantities of radioactivity in Put, Spd and Spm after 20 h of incubation. The effects of glycerol on growth were inhibited by the ornithine decarboxylase (EC 4.1.1.17) inhibitor α‐difluoromethylornithine (DFMO). The presence of DFMO in the incubation medium with [U‐14C]‐glycerol also reduced the radioactivity in PAs.
URI: http://hdl.handle.net/10553/46483
ISSN: 0031-9317
DOI: 10.1034/j.1399-3054.2000.1100416.x
Fuente: Physiologia Plantarum [ISSN 0031-9317], v. 110 (4), p. 530-534
Colección:Artículos
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