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Title: Differentiation of embryonic stem cells using pancreatic bud-conditioned medium gives rise to neuroectoderm-derived insulin-secreting cells
Authors: Vicente-Salar, Nestor
Santana, Alfredo 
Reig, Juan A.
Roche, Enrique
UNESCO Clasification: 32 Ciencias médicas
241003 Citología humana
2407 Biología celular
Keywords: Embryonic stem cells
Pancreatic bud
Definitive endoderm
Germ layers
Issue Date: 2011
Journal: Cellular Reprogramming 
Abstract: Human embryonic stem cells can be differentiated into insulin-secreting cells by emulating in vitro the key processes that occur during embryonic development. However, the resulting cells are generally immature; thus, further research must be performed to identify the necessary factors to complete the differentiation. To this end, we cultured mouse embryonic stem cells with pancreatic bud-conditioned medium, based on a recent publication. Unlike in humans, mouse cells present two types of insulin, which can be used to identify different cell lineages. As a result, the cell product presented a neuroectodermal genetic expression pattern, with no expression of any definitive endodermal marker analyzed. Also, nonglucose-dependent insulin release was detected. Altogether, this previously published protocol results in neuroectoderm, and not definitive endoderm, derived insulin-positive cells. This further confirms the difficulty of obtaining true cell types of this germ layer. Finally, we identified a 16-kDa protein band that was present in pancreatic bud-conditioned medium. Sequencing this band revealed the presence of Reg proteins. The role of pancreatic bud-conditioned medium remains to be tested in definitive endoderm committed cells.
ISSN: 2152-4971
DOI: 10.1089/cell.2010.0054
Source: Cellular Reprogramming [ISSN 2152-4971],v. 13, p. 77-84
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