Please use this identifier to cite or link to this item:
Title: Negative neuronal differentiation of human adipose-derived stem cell clones
Authors: Arribas, María I.
Ropero, Ana B.
Reig, Juan A.
Fraga, Mario F.
Fernandez, Agustin F.
Santana, Alfredo 
Roche, Enrique
UNESCO Clasification: 32 Ciencias médicas
320507 Neurología
2407 Biología celular
Keywords: Adipose tissue
Adult stem cells
Cell therapy
Neurodegenerative diseases
Issue Date: 2014
Journal: Regenerative Medicine 
Abstract: Aims: Adipose mesenchymal stem cells are a heterogeneous population. Therefore, the question posed in this study is whether the heterogenic differentiation potential exhibited by the different clones toward mesodermic lineages can be extended to nonmesodermic lineages, such as the neuroectoderm. Materials & methods: Different single cell clones of human adipose mesenchymal stem cells from the same donor were isolated. Neuronal plasticity of the clones was assessed according to the pattern DNA methylation, gene expression and intracellular calcium responses. Results: Under neurogenic culture conditions, clones presented variable expression of neuronal-specific genes, but still expressed osteogenic markers. No calcium response was exhibited in response to KCl incubation. The DNA methylation profile presented a very similar pattern in neuroectoderm gene promoters. Conclusions: Data indicate that there are no significant differences between the undifferentiated and supposedly neuronal-differentiated mesenchymal cells.
ISSN: 1746-0751
DOI: 10.2217/rme.14.11
Source: Regenerative Medicine [ISSN 1746-0751], v. 9, p. 279-293
Appears in Collections:Artículos
Show full item record

Google ScholarTM




Export metadata

Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.