Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/45936
Title: Candida albicans Germ-Tube Antibody: Evaluation of a New Automatic Assay for Diagnosing Invasive Candidiasis in ICU Patients
Authors: Parra-Sánchez, Manuel
Zakariya-Yousef Breval, Ismail
Castro Méndez, Carmen
García-Rey, Silvia
Loza Vazquez, Ana
Úbeda Iglesias, Alejandro
Macías Guerrero, Desiree
Romero Mejías, Ana
León Gil, Cristobal
Martín-Mazuelos, Estrella
Tomás, J. I.
Gurpegui, M.
González, V.
Rezusta, A.
Úbeda, A.
Rodríguez, A.
Bodi, M.
Gómez, F.
Trefler, S.
Llauradó, M.
Güell, R.
González, R.
Díaz, F. J.
González, Raúl
Marcos, J. M.
Carriedo, D.
Fernández, I.
Valverde, E.
Esteban, A.
Marrodan, T.
Arenzana, A.
González, C.
Suárez, A. I.
Ballús, J.
Esteve, F.
Ayats, J.
Aguilar, G.
Badenes, R.
Ferrando, C.
Navarro, D.
Carbonell, J. A.
García, C.
Gómez, A.
Henao, L.
Miñana, A.
Puig, J.
Belda, F. J.
Puppo, A.
Garnacho, J.
Márquez, J. A.
Gutiérrez, A.
Ruiz, M.
Jeréz, V.
Trasmonte, V.
Martínez, P.
Pérez, D.
Fajardo, M.
Gaona, C.
Muñoz, C.
Tamayo, G.
Martínez, A.
Labayen, F.
Iruretagoyena, J. R.
López, L.
Hernández, J. L.
Ruiz-Santana, S. 
Hernández, M. A.
Bordes, A.
Ojeda, N.
Jiménez, J. D.
UNESCO Clasification: 32 Ciencias médicas
3207 Patología
Keywords: Candida albicans germ-tube antibody (CAGTA)
Critically ill patients
ICU
Invasive candidiasis
Serum diagnostic test
Issue Date: 2017
Journal: Mycopathologia 
Abstract: Testing for Candida albicans germ-tube antibody IFA IgG assay (CAGTA) is used to detect invasive candidiasis infection. However, most suitable assays lack automation and rapid single-sample testing. The CAGTA assay was adapted in an automatic monotest system (invasive candidiasis [CAGTA] VirClia® IgG monotest (VirClia®), a chemiluminescence assay with ready-to-use reagents that provides a rapid objective result. CAGTA assay was compared with the monotest automatic VirClia® assay in order to establish the diagnostic reliability, accuracy, and usefulness of this method. A prospective study with 361 samples from 179 non-neutropenic critically ill adults patients was conducted, including 21 patients with candidemia, 18 with intra-abdominal candidiasis, 84 with Candida spp. colonization, and 56 with culture-negative samples, as well as samples from ten healthy subjects. Overall agreement between the two assays (CAGTA and VirCLIA) was 85.3%. These assays were compared with the gold-standard method to determine the sensitivity, specificity as well as positive and negative predictive values. In patients with candidemia, values for CAGTA and VirCLIA assays were 76.2 versus 85.7%, 80.3 versus 75.8%, 55.2 versus 52.9%, and 91.4 versus 94.3%, respectively. The corresponding values in patients with intra-abdominal candidiasis were 61.1 versus 66.7%, 80.3 versus 75.8%, 45.8 versus 42.9%, and 88.3 versus 89.3%, respectively. No differences were found according to the species of Candida isolated in culture, except for Candida albicans and C. parapsilosis, for which VirClia® was better than CAGTA. According to these results, the automated VirClia® assay was a reliable, rapid, and very easy to perform technique as tool for the diagnosis invasive candidiasis.
URI: http://hdl.handle.net/10553/45936
ISSN: 0301-486X
DOI: 10.1007/s11046-017-0125-9
Source: Mycopathologia [ISSN 0301-486X], v. 182 (7-8), p. 645-652
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