Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/4592
Title: Characterization of polysaccharides from Hypnea spinella (Gigartinales) and Halopithys incurva (Ceramiales) and their effect on RAW 264.7 macrophage activity
Authors: Abdala Díaz, Roberto T.
Chabrillón, Mariana
Cabello-Pasini, Alejandro
Gómez-Pinchetti, Juan Luis 
Figueroa, Félix L.
UNESCO Clasification: 241707 Algología (ficología)
Keywords: Polysaccharides
Immunomodulator
Macrophages
FT-IR spectroscopy
Issue Date: 2010
Abstract: Red algae have been reported to be an important source of polysaccharides with potential immunomodulatory properties. The objective of this study was to characterize the polysaccharides from Halopithys incurva and Hypnea spinella and to evaluate their effect on the synthesis of cytokines by murine cell line RAW 264.7 macrophages. Polysaccharides were obtained by N-cetylpyridiniumbromide precipitation and characterized by Fourier transform-infrared spectroscopy. Their effect on the activity of RAW 264.7 macrophages was examined by quantification of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and nitric oxide (NO) production using enzyme-linked immunosorbent assays. The activation of the cytokine IL-6 and NO increased linearly as the concentration of polysaccharides from H. incurva and Hy. spinella increased. In general, the activation of IL-6 and NO was tenfold greater when macrophages were exposed to polysaccharides from H. incurva than when exposed to polysaccharides from Hy. spinella. In contrast, TNF-α concentration did not increase when macrophages\nwere exposed to increasing polysaccharide levels. These\nresults indicate that polysaccharides are strong cytokine IL-6 inducers.
URI: http://hdl.handle.net/10553/4592
ISSN: 0921-8971
DOI: 10.1007/s10811-010-9622-7
Source: Journal of Applied Phycology. Netherlands: Springer, 2010 [ISSN 0921-8971], v. 23 (3)
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