|Title:||Methodology for in situ gas sampling, transport and laboratory analysis of gases from stranded cetaceans||Authors:||Bernaldo de Quirós Miranda, Yara
González Díaz, Oscar Manuel
Saavedra Santana, Pedro
Arbelo Hernández, Manuel Antonio
Sierra Pulpillo, Eva María
Sacchini , Simona
Jepson, Paul D.
Di Guardo, Giovanni
Fernández Rodríguez, Antonio Jesús
|UNESCO Clasification:||310907 Patología||Keywords:||Gas-bubble lesions
|Issue Date:||2011||Journal:||Scientific Reports||Abstract:||Gas-bubble lesions were described in cetaceans stranded in spatio-temporal concordance with naval exercises using high-powered sonars. A behaviourally induced decompression sickness-like disease was proposed as a plausible causal mechanism, although these findings remain scientifically controversial. Investigations into the constituents of the gas bubbles in suspected gas embolism cases are highly desirable. We have found that vacuum tubes, insulin syringes and an aspirometer are reliable tools for in situ gas sampling, storage and transportation without appreciable loss of gas and without compromising the accuracy of the analysis. Gas analysis is conducted by gas chromatography in the laboratory. This methodology was successfully applied to a mass stranding of sperm whales, to a beaked whale stranded in spatial and temporal association with military exercises and to a cetacean chronic gas embolism case. Results from the freshest animals confirmed that bubbles were relatively free of gases associated with putrefaction and consisted predominantly of nitrogen.||URI:||http://hdl.handle.net/10553/45813||ISSN:||2045-2322||DOI:||10.1038/srep00193||Source:||Scientific Reports [ISSN 2045-2322], v. 1|
|Appears in Collections:||Artículos|
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