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Title: Methodology for in situ gas sampling, transport and laboratory analysis of gases from stranded cetaceans
Authors: Bernaldo de Quirós Miranda, Yara 
González Díaz, Oscar Manuel 
Saavedra Santana, Pedro 
Arbelo Hernández, Manuel Antonio 
Sierra Pulpillo, Eva María 
Sacchini , Simona 
Jepson, Paul D.
Mazzariol, Sandro
Di Guardo, Giovanni
Fernández Rodríguez, Antonio Jesús 
UNESCO Clasification: 310907 Patología
Keywords: Gas-bubble lesions
Military maneuvers
Stranded cetaceans
Issue Date: 2011
Journal: Scientific Reports 
Abstract: Gas-bubble lesions were described in cetaceans stranded in spatio-temporal concordance with naval exercises using high-powered sonars. A behaviourally induced decompression sickness-like disease was proposed as a plausible causal mechanism, although these findings remain scientifically controversial. Investigations into the constituents of the gas bubbles in suspected gas embolism cases are highly desirable. We have found that vacuum tubes, insulin syringes and an aspirometer are reliable tools for in situ gas sampling, storage and transportation without appreciable loss of gas and without compromising the accuracy of the analysis. Gas analysis is conducted by gas chromatography in the laboratory. This methodology was successfully applied to a mass stranding of sperm whales, to a beaked whale stranded in spatial and temporal association with military exercises and to a cetacean chronic gas embolism case. Results from the freshest animals confirmed that bubbles were relatively free of gases associated with putrefaction and consisted predominantly of nitrogen.
ISSN: 2045-2322
DOI: 10.1038/srep00193
Source: Scientific Reports [ISSN 2045-2322], v. 1
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