Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/45697
Title: Ancient DNA in archaeological wheat grains: Preservation conditions and the study of pre-Hispanic agriculture on the island of Gran Canaria (Spain)
Authors: Oliveira, Hugo R.
Civáň, Peter
Morales Mateos, Jacob Bentejui 
Rodríguez Rodríguez, Amelia Del Carmen 
Lister, Diane L.
Jones, Martin K.
UNESCO Clasification: 550405 Prehistoria
550501 Arqueología
Keywords: Wheat
Ancient DNA
Preservation
Microsatellites
Canary Islands, et al
Issue Date: 2012
Publisher: 0305-4403
Journal: Journal of Archaeological Science 
Abstract: The recovery of ancient DNA from archaeological wheat samples under different preservation conditions was assessed using a number of genetic markers. It was possible to amplify nuclear DNA from desiccated grains but not from charred. The desiccated grain was from a pre-Hispanic grain silo in Gran Canaria and showed excellent DNA preservation, enabling the amplification of the ribosomal DNA markers IGS and ITS, the upstream region of the HMW-glutenin locus and single-locus nuclear microsatellites. Our results demonstrated the presence of both durum and bread wheat in an assemblage of naked grain. We were also able to identify different genotypes in durum wheat and compare these with extant landraces, providing insights into the agrarian practices of the ancient Canarians and the origin of their crops.
URI: http://hdl.handle.net/10553/45697
ISSN: 0305-4403
DOI: 10.1016/j.jas.2011.10.008
Source: Journal Of Archaeological Science[ISSN 0305-4403],v. 39 (4), p. 828-835
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