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Title: Molecular and chemical characterization of a Sphagnum palustre clone: key steps towards a standardized and sustainable moss bag technique
Authors: Di Palma, A.
Crespo Pardo, D.
Spagnuolo, V.
Adamo, P.
Bargagli, R.
Cafasso, D.
Capozzi, F.
Aboal, J. R.
González, A. G. 
Pokrovsky, O.
Beike, A. K.
Reski, R.
Tretiach, M.
Varela, Z.
Giordano, S.
UNESCO Clasification: 2417 Biología vegetal (botánica)
Keywords: Atmospheric pollution
Moss bags
Trace elements
DNA molecular markers
Issue Date: 2016
Project: Changes in carbon uptake and emissions by oceans in a changing climate ItemCrisRefDisplayStrategy.project.deleted.icon
Journal: Ecological Indicators 
Abstract: This work aimed to define the molecular and chemical signature of a S. palustre clone developed in the framework of the EU-FP7 Mossclone project to improve the standardization and reliability of the moss-bag technique. The molecular characterization was performed by a set of DNA molecular markers (RAPD, ISJ, PCR-RFLP, sequencing and microsatellites) to tag the clone produced within the project. Molecular characterization also provided new DNA markers that can be applied in systematic analyses of Sphagnum, and gave new insights to implement well established techniques. The elemental composition of the clone was measured by ICP-MS analysis of 54 major and trace elements, with and without commonly applied pre-exposure treatments (oven devitalization and EDTA washing). Concentrations of almost all analyzed elements were significantly lower (from 10 to 100 times) in the clone than in conspecific field moss, apart from some elements (K, Mo, P and Na) deriving from the culture medium or EDTA treatment. Oven devitalization and EDTA washing did not significantly affect the clone composition. A comparison between the elemental composition of the clone with that of naturally growing Sphagnum species proved the particularly low elemental content of the clone. Therefore, in view of a rigorously standardized moss-bag protocol for the monitoring of persistent atmospheric pollutants, the use of the S. palustre clone, a biomaterial with very low and constant element composition, and homogenous morphological characteristics is strongly recommended.
ISSN: 1470-160X
DOI: 10.1016/j.ecolind.2016.06.044
Source: Ecological Indicators [ISSN 1470-160X], v. 71, p. 388-397
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