Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/45514
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dc.contributor.authorRomero-Aleman, M. M.
dc.contributor.authorMonzón-Mayor, M.
dc.contributor.authorSantos, E.
dc.contributor.authorYanes, C.
dc.contributor.otherROMERO-ALEMAN, MARIA DEL MAR
dc.contributor.otherMonzon-Mayor, Maximina
dc.date.accessioned2018-11-22T10:26:35Z-
dc.date.available2018-11-22T10:26:35Z-
dc.date.issued2010
dc.identifier.issn0021-9967
dc.identifier.urihttp://hdl.handle.net/10553/45514-
dc.description.abstractSpontaneous regrowth of retinal ganglion cell (RGC) axons occurs after optic nerve (ON) transection in the lizard Gallotia galloti. To gain more insight into this event we performed an immunohistochemical study on selected neuron and glial markers, which proved useful for analyzing the axonal regrowth process in different regeneration models. In the control lizards, RGCs were beta-III tubulin- (Tuj1) and HuCD-positive. The vesicular glutamate transporter-1 (VGLUT1) preferentially stained RGCs and glial somata rather than synaptic layers. In contrast, SV2 and vesicular GABA/glycine transporter (VGAT) labeling was restricted to both plexiform layers. Strikingly, the strong expression of glutamine synthetase (GS) in both Muller glia processes and macroglial somata revealed a high glutamate metabolism along the visual system. Upregulation of Tuj1 and HuCD in the surviving RGCs was observed at all the timepoints studied (1, 3, 6, 9, and 12 months postlesion). The significant rise of Tuj1 in the optic nerve head and optic tract (OTr) by 1 and 6 months postlesion, respectively, suggests an increase of the beta-III tubulin transport and incorporation into newly formed axons. Persistent Tuj1(+) and SV2(+) puncta and swellings were abnormally observed in putative degenerating/dystrophic fibers. Unexpectedly, neuron-like cells of obscure significance were identified in the control and regenerating ON-OTr. We conclude that: 1) the persistent upregulation of Tuj1 and HuCD favors the long-lasting axonal regrowth process; 2) the latter succeeded despite the ectopia and dystrophy of some regrowing fibers; and 3) maintenance of the glutamate-glutamine cycle contributes to the homeostasis and plasticity of the system. J. Comp. Neurol. 518:4067-4087, 2010. (C) 2010 Wiley-Liss, Inc.
dc.publisher0021-9967
dc.relation.ispartofJournal of Comparative Neurology
dc.sourceJournal Of Comparative Neurology[ISSN 0021-9967],v. 518 (19), p. 4067-4087
dc.subject.otherRetinal Ganglion-Cells
dc.subject.otherFibrillary Acidic Protein
dc.subject.otherOptic-Nerve Regeneration
dc.subject.otherGallotia-Galloti
dc.subject.otherGlial-Cells
dc.subject.otherMessenger-Rna
dc.subject.otherWhite-Matter
dc.subject.otherAxon Growth
dc.subject.otherOligodendrocyte Differentiation
dc.subject.otherNeurotrophic Factor
dc.titleExpression of neuronal markers, synaptic proteins, and glutamine synthetase in the control and regenerating lizard visual system
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1002/cne.22444
dc.identifier.scopus77955695838-
dc.identifier.isi000281331000010
dcterms.isPartOfJournal Of Comparative Neurology
dcterms.sourceJournal Of Comparative Neurology[ISSN 0021-9967],v. 518 (19), p. 4067-4087
dc.contributor.authorscopusid6506533545
dc.contributor.authorscopusid36793900900
dc.contributor.authorscopusid35084324600
dc.contributor.authorscopusid7004187530
dc.description.lastpage4087
dc.description.firstpage4067
dc.relation.volume518
dc.type2Artículoes
dc.identifier.wosWOS:000281331000010
dc.contributor.daisngid1157526
dc.contributor.daisngid901526
dc.contributor.daisngid2816094
dc.contributor.daisngid675718
dc.identifier.investigatorRIDK-8038-2014
dc.identifier.investigatorRIDNo ID
dc.contributor.wosstandardWOS:Romero-Aleman, MM
dc.contributor.wosstandardWOS:Monzon-Mayor, M
dc.contributor.wosstandardWOS:Santos, E
dc.contributor.wosstandardWOS:Yanes, C
dc.date.coverdateOctubre 2010
dc.identifier.ulpgces
dc.description.jcr3,774
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUIBS: Farmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.deptGIR IUIBS: Tecnología Médica y Audiovisual-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.orcid0000-0002-7987-5509-
crisitem.author.orcid0000-0002-5046-508X-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameRomero Alemán, María Del Mar-
crisitem.author.fullNameMonzón Mayor,Maximina-
crisitem.author.fullNameYanes Mendez, Carmen M-
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