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http://hdl.handle.net/10553/45511
Título: | Expression of BDNF and NT-3 during the ontogeny and regeneration of the lacertidian (Gallotia galloti) visual system | Autores/as: | Santos, E. Romero-Aleman, M. M. Monzón-Mayor, M. Lang, D. M. Rodger, J. Yanes, C. |
Palabras clave: | Retinal Ganglion-Cells Optic-Nerve Regeneration Neurotrophic Factor Bdnf Messenger-Rna Glutamine-Synthetase, et al. |
Fecha de publicación: | 2011 | Editor/a: | 1932-8451 | Publicación seriada: | Developmental Neurobiology | Resumen: | Retinal ganglion cell (RGC) axons regrow spontaneously after optic nerve (ON) transection in G. galloti. Because brain-derived neurotrophic factor (BDNF) is considered the major neurotrophin participating in vertebrate visual system development and promotes RGC survival, we investigated its distribution using dual-labeling immunohistochemistry for neuronal and glial markers. We examined the developing and regenerating lizard visual system at 1, 3, 6, 9, and 12 months postlesion to comparatively evaluate BDNF expression patterns. BDNF was detected from midembryonic stages (E35) in both retinal plexiform layers, and in radial glial processes in the tectum. Moreover, RGC axon staining was detected at late prenatal stages (E39), showing a transient punctate staining which progressed in a temporo-spatial pattern that was similar to myelination. Strong expression in RGC axons was maintained in adults. However, transient downregulation of BDNF staining occurred on the experimental side one month after ON transection followed by a gradual recovery with extensive punctate/swelling distribution and persistent upregulation at 12 months. Conversely, quantitative PCR analysis for 1 and 12 months regenerate lizards showed downregulation of the ratio of BDNF mRNA expression at 12 months and nonsignificant changes of NT-3 transcripts. In summary, we demonstrate that BDNF and NT-3 are abundantly expressed during lizard visual system ontogeny and regeneration suggesting their participation in the development, maintenance and plasticity of the system. (C) 2011 Wiley Periodicals, Inc. Develop Neurobiol 71: 836-853, 2011 | URI: | http://hdl.handle.net/10553/45511 | ISSN: | 1932-8451 | DOI: | 10.1002/dneu.20939 | Fuente: | Developmental Neurobiology[ISSN 1932-8451],v. 71 (10), p. 836-853 |
Colección: | Artículos |
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