Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/45507
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dc.contributor.authorHernández-Morera, Pabloen_US
dc.contributor.authorCastaño-González, Ireneen_US
dc.contributor.authorTravieso-González, Carlos M.en_US
dc.contributor.authorMompeó Corredera, Blanca Rosaen_US
dc.contributor.authorOrtega-Santana, Franciscoen_US
dc.date.accessioned2018-11-22T10:23:11Z-
dc.date.available2018-11-22T10:23:11Z-
dc.date.issued2016en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/10553/45507-
dc.description.abstractPurpose To develop a digital image processing method to quantify structural components (smooth muscle fibers and extracellular matrix) in the vessel wall stained with Masson’s trichrome, and a statistical method suitable for small sample sizes to analyze the results previously obtained. Methods The quantification method comprises two stages. The pre-processing stage improves tissue image appearance and the vessel wall area is delimited. In the feature extraction stage, the vessel wall components are segmented by grouping pixels with a similar color. The area of each component is calculated by normalizing the number of pixels of each group by the vessel wall area. Statistical analyses are implemented by permutation tests, based on resampling without replacement from the set of the observed data to obtain a sampling distribution of an estimator. The implementation can be parallelized on a multicore machine to reduce execution time. Results The methods have been tested on 48 vessel wall samples of the internal saphenous vein stained with Masson’s trichrome. The results show that the segmented areas are consistent with the perception of a team of doctors and demonstrate good correlation between the expert judgments and the measured parameters for evaluating vessel wall changes. Conclusion The proposed methodology offers a powerful tool to quantify some components of the vessel wall. It is more objective, sensitive and accurate than the biochemical and qualitative methods traditionally used. The permutation tests are suitable statistical techniques to analyze the numerical measurements obtained when the underlying assumptions of the other statistical techniques are not met.en_US
dc.languageengen_US
dc.relation.ispartofPLoS ONEen_US
dc.sourcePLoS ONE [ISSN 1932-6203],v. 11 (1), e0146954en_US
dc.subject32 Ciencias médicasen_US
dc.subject240401 Bioestadísticaen_US
dc.subject.otherMasson's Trichromeen_US
dc.subject.otherSmooth muscleen_US
dc.subject.otherDigital image proceesingen_US
dc.subject.otherEstimation theoryen_US
dc.titleQuantification and statistical analysis methods for vessel wall components from stained images with Masson's trichromeen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0146954en_US
dc.identifier.scopus2-s2.0-84955308846-
dc.contributor.authorscopusid36782546400-
dc.contributor.authorscopusid57073688900-
dc.contributor.authorscopusid6602376272-
dc.contributor.authorscopusid57073831000-
dc.contributor.authorscopusid6506142524-
dc.identifier.issue0146954-
dc.relation.volume11en_US
dc.investigacionIngeniería y Arquitecturaen_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-TELen_US
dc.description.sjr1,201
dc.description.jcr2,806
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
dc.description.erihplusERIH PLUS
item.grantfulltextopen-
item.fulltextCon texto completo-
crisitem.author.deptGIR IUMA: Sistemas de Información y Comunicaciones-
crisitem.author.deptIU de Microelectrónica Aplicada-
crisitem.author.deptDepartamento de Ingeniería Telemática-
crisitem.author.deptGIR IDeTIC: División de Procesado Digital de Señales-
crisitem.author.deptIU para el Desarrollo Tecnológico y la Innovación-
crisitem.author.deptDepartamento de Señales y Comunicaciones-
crisitem.author.deptGIR IUSA-ONEHEALTH 3: Histología y Patología Veterinaria y Forense (Terrestre y Marina)-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.orcid0000-0002-0907-4765-
crisitem.author.orcid0000-0002-4621-2768-
crisitem.author.orcid0000-0003-4953-7653-
crisitem.author.orcid0000-0001-9041-153X-
crisitem.author.parentorgIU de Microelectrónica Aplicada-
crisitem.author.parentorgIU para el Desarrollo Tecnológico y la Innovación-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameHernández Morera, Pablo Vicente-
crisitem.author.fullNameTravieso González, Carlos Manuel-
crisitem.author.fullNameMompeó Corredera, Blanca Rosa-
crisitem.author.fullNameOrtega Santana, Francisco Cándido-
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