Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/45363
Título: Excretory/secretory antigens from Dirofilaria immitis adult worms interact with the host fibrinolytic system involving the vascular endothelium
Autores/as: González-Miguel, Javier
Morchon Garcia,Rodrigo 
Mellado, Isabel
Carretón Gomez, Elena 
Montoya Alonso, José Alberto 
Simón Martín,Fernando 
Clasificación UNESCO: 240112 Parasitología animal
310907 Patología
Palabras clave: Dirofilaria immitis
Excretory/secretory antigens
Plasminogen binding
t-PA
Endothelial cells, et al.
Fecha de publicación: 2012
Editor/a: 0166-6851
Publicación seriada: Molecular and Biochemical Parasitology 
Resumen: Dirofilaria immitis is the causative agent of canine and feline heartworm disease. The parasite can survive for long periods of time (7 years or more) in the circulatory system of immunocompetent reservoirs, producing usually a chronic inflammatory vascular disease. In addition, the simultaneous death of groups of adult worms can trigger an acute disease characterized by the exacerbation of inflammatory reactions and the emergence of serious thromboembolic events. In the context of the D. immitis/host relationships, the aim of this study was to investigate the interaction between the excretory/secretory antigens from D. immitis adult worms (DiES) and the fibrinolytic system of the host. Using an enzyme-linked immunosorbent assay we showed that DiES extract is able to bind plasminogen and generate plasmin, although this fact requires the presence of the tissue plasminogen activator (t-PA). Moreover, we established that DiES extract enhances t-PA expression in cultured vascular endothelial cells. Additionally, 10 plasminogen-binding proteins from DiES extract were identified by mass spectrometry (HSP60, actin-1/3, actin, actin 4, transglutaminase, GAPDH, Ov87, LOAG_14743, galectin and P22U). The data suggest that DiES antigens interact with the environment of the parasite regulating the activation of the fibrinolytic system of the host with involvement of the vascular endothelium in the process.
URI: http://hdl.handle.net/10553/45363
ISSN: 0166-6851
DOI: 10.1016/j.molbiopara.2011.10.010
Fuente: Molecular and Biochemical Parasitology [ISSN 0166-6851], v. 181, p. 134-140
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