Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/45335
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dc.contributor.authorGonzález-Antuña, Anaen_US
dc.contributor.authorRodríguez-González, Pabloen_US
dc.contributor.authorCentineo, Giuseppeen_US
dc.contributor.authorGarcía Alonso, J. Ignacioen_US
dc.date.accessioned2018-11-22T09:02:16Z-
dc.date.available2018-11-22T09:02:16Z-
dc.date.issued2010en_US
dc.identifier.issn0003-2654en_US
dc.identifier.urihttp://hdl.handle.net/10553/45335-
dc.description.abstractA procedure for Stable Isotope Dilution Analysis in molecular Mass Spectrometry which does not require a methodological calibration graph and can be applied in combination with minimal labelling has been evaluated. This alternative approach is based on the determination of the molar fractions for each pure isotope pattern (natural abundance or labelled) contributing to the isotope pattern observed in the mixture of natural abundance and labelled molecules by multiple linear regression. Two labelled compounds, (13)C(1)-labelled or (13)C(6)-labelled phenol, were compared to study the influence of the number of (13)C atoms in the labelled molecule. The procedure was evaluated by comparing the results obtained for the determination of phenol in NIST 1584 CRM by GC-EI-MS using the classical isotope dilution calibration procedure and the new procedure based on multiple linear regression of isotope patterns without a calibration graph. The results obtained using the proposed procedure agreed well with both the certified values and those obtained using the classical Isotope Dilution Mass Spectrometry (IDMS) calibration procedures. For the evaluated procedure, a full uncertainty budget determination has been developed taking into account all uncertainty sources, including those derived from the uncertainties in the isotope patterns of the natural and labelled compounds. The measurements with the (13)C(1)-labelled phenol provided lower propagated uncertainties in comparison to the use of (13)C(6)-labelled phenol.en_US
dc.languageengen_US
dc.relation.ispartofAnalyst (London. 1877. Print)en_US
dc.sourceAnalyst [ISSN 0003-2654],v. 135 (5), p. 953-964 (Mayo 2010)en_US
dc.subject32 Ciencias médicasen_US
dc.subject2301 química analíticaen_US
dc.subject.other13C-labellingen_US
dc.subject.otherIsotope dilutionen_US
dc.subject.otherOrganic analysisen_US
dc.titleEvaluation of minimal 13C-labelling for stable isotope dilution in organic analysisen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1039/b924432hen_US
dc.identifier.scopus77951580545-
dc.contributor.authorscopusid36058862700-
dc.contributor.authorscopusid6603228633-
dc.contributor.authorscopusid35557054000-
dc.contributor.authorscopusid7005508838-
dc.description.lastpage964en_US
dc.identifier.issue5-
dc.description.firstpage953en_US
dc.relation.volume135en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages12en_US
dc.utils.revisionen_US
dc.date.coverdateMayo 2010en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr3,913
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.fullNameGonzález Antuña, Ana-
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