Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/45335
Title: Evaluation of minimal 13C-labelling for stable isotope dilution in organic analysis
Authors: González-Antuña, Ana 
Rodríguez-González, Pablo
Centineo, Giuseppe
García Alonso, J. Ignacio
UNESCO Clasification: 32 Ciencias médicas
2301 química analítica
Keywords: 13C-labelling
Isotope dilution
Organic analysis
Issue Date: 2010
Journal: Analyst (London. 1877. Print) 
Abstract: A procedure for Stable Isotope Dilution Analysis in molecular Mass Spectrometry which does not require a methodological calibration graph and can be applied in combination with minimal labelling has been evaluated. This alternative approach is based on the determination of the molar fractions for each pure isotope pattern (natural abundance or labelled) contributing to the isotope pattern observed in the mixture of natural abundance and labelled molecules by multiple linear regression. Two labelled compounds, (13)C(1)-labelled or (13)C(6)-labelled phenol, were compared to study the influence of the number of (13)C atoms in the labelled molecule. The procedure was evaluated by comparing the results obtained for the determination of phenol in NIST 1584 CRM by GC-EI-MS using the classical isotope dilution calibration procedure and the new procedure based on multiple linear regression of isotope patterns without a calibration graph. The results obtained using the proposed procedure agreed well with both the certified values and those obtained using the classical Isotope Dilution Mass Spectrometry (IDMS) calibration procedures. For the evaluated procedure, a full uncertainty budget determination has been developed taking into account all uncertainty sources, including those derived from the uncertainties in the isotope patterns of the natural and labelled compounds. The measurements with the (13)C(1)-labelled phenol provided lower propagated uncertainties in comparison to the use of (13)C(6)-labelled phenol.
URI: http://hdl.handle.net/10553/45335
ISSN: 0003-2654
DOI: 10.1039/b924432h
Source: Analyst [ISSN 0003-2654],v. 135 (5), p. 953-964 (Mayo 2010)
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