Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/45331
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dc.contributor.authorGonzález-Antuña, Anaen_US
dc.contributor.authorRodríguez-González, Pabloen_US
dc.contributor.authorCentineo, Giuseppeen_US
dc.contributor.authorGarcía Alonso, J. Ignacioen_US
dc.date.accessioned2018-11-22T09:00:26Z-
dc.date.available2018-11-22T09:00:26Z-
dc.date.issued2014en_US
dc.identifier.issn0021-9673en_US
dc.identifier.urihttp://hdl.handle.net/10553/45331-
dc.description.abstractSeven β2-agonist (clenproperol, clenbuterol, salbutamol, bronbuterol, ractopamine, clenpenterol and clencyclohexerol) were determined simultaneously in human and bovine urine by isotope dilution LC-ESI-MS/MS in a triple quadrupole instrument. The method is based on the application of multiple linear regression in combination with compound-specific minimally 13C-labelled analogues. Additionally, the increase of the bandpass of the first quadrupole during the selected reaction monitoring (SRM) measurement procedure allowed the simultaneous quantification of the seven compounds at sub ngg-1 levels in a single chromatogram without resorting to a methodological calibration graph. Recovery values at concentration levels between 5.0 and 0.05ngg-1 ranged from 95 to 110% in fortified bovine urine and from 91 to 108% in human urine, with relative standard deviations lower than 5% except for salbutamol and ractopamine. The proposed methodology was validated by analyzing the certified reference material BCR-503 (lyophilized bovine urine) certified for clenbuterol and salbutamol. The limits of detection (LOD) for a sample volume of 10mL of both human and bovine urine was found to be lower than 0.012ngg-1 for all compounds, except to salbutamol in bovine urine which was of 0.029ngg-1. The use of compound-specific isotopically labelled analogues minimally labelled in 13C minimized the occurrence of isotope effects and corrected for matrix effects during ESI ionization and can be efficiently applied for the quantification of ultra-trace concentrations of β2-agonists in human and bovine urine.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Chromatography Aen_US
dc.sourceJournal of Chromatography A [ISSN 0021-9673], v. 1372, p. 63-71en_US
dc.subject32 Ciencias médicasen_US
dc.subject230103 Análisis cromatográficoen_US
dc.subject230110 Espectroscopia de masasen_US
dc.subject.otherIsotope dilutionen_US
dc.subject.otherLiquid chromatographyen_US
dc.subject.otherMatrix effectsen_US
dc.subject.otherMinimal labelingen_US
dc.subject.otherSelected reaction monitoringen_US
dc.subject.otherTandem mass spectrometryen_US
dc.titleSimultaneous determination of seven β2-agonists in human and bovine urine by isotope dilution liquid chromatography-tandem mass spectrometry using compound-specific minimally 13C-labelled analoguesen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.chroma.2014.10.065en_US
dc.identifier.scopus84915763953-
dc.contributor.authorscopusid36058862700-
dc.contributor.authorscopusid6603228633-
dc.contributor.authorscopusid35557054000-
dc.contributor.authorscopusid7005508838-
dc.description.lastpage71en_US
dc.description.firstpage63en_US
dc.relation.volume1372en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages9en_US
dc.utils.revisionen_US
dc.date.coverdateDiciembre 2014en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr1,825
dc.description.jcr4,169
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.fullNameGonzález Antuña, Ana-
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