Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/45306
Title: Inositol-phosphoglycan inhibits calcium oscillations in hepatocytes by reducing calcium entry
Authors: Sanchez-Bueno, Antonio
Greenwood, Mark R.
Varela-Nieto, Isabel
Marrero Arencibia, María Isabel 
Gil, Beatriz
Mato, Jose M.
Cobbold, Peter H.
UNESCO Clasification: 32 Ciencias médicas
320502 Endocrinología
Keywords: Inositol-phosphoglycan
Calcium
Hepatocytes
Issue Date: 1997
Journal: Cell Calcium 
Abstract: Inositol-phosphoglycan (IPG) is a putative mediator of insulin action that has been shown to affect numerous biochemical processes. IPG, prepared from liver membranes, promptly inhibited phenylephrine- or vasopressin-induced [Ca2+]i oscillations when perfused over Fura-2-dextran injected rat hepatocytes. An antibody to IPG suppressed the inhibitory effect of insulin on the [Ca2+]i oscillations. Measurement of the rate of quench of cytoplasmic Fura-2 by extracellular Mn2+ showed that Ca2+ entry occurred continuously in the unstimulated cell and was not affected by phenylephrine or vasopressin. IPG, specifically, almost completely abolished the Mn2+ quench rate. Elevated extracellular [Ca2+] reversed the inhibitory effect of IPG on [Ca2+]i oscillations. We conclude that IPG inhibits the hepatocyte Ca2+ oscillatory by reducing the continuous Ca2+ influx that is required to sustain oscillations in [Ca2+]i.
URI: http://hdl.handle.net/10553/45306
ISSN: 0143-4160
DOI: 10.1016/S0143-4160(97)90036-1
Source: Cell Calcium [ISSN 0143-4160], v. 21 (2), p. 125-133
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