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Title: Leptin signaling in skeletal muscle after bed rest in healthy humans
Authors: Guerra Hernández, Carlos Borja 
Ponce González,Jesús Gustavo 
Morales-Alamo, David 
Guadalupe Grau, Amelia 
Kiilerich, Kristian
Fuentes, Teresa 
Ringholm, Stine
Biensø, Rasmus Sjørup
Santana, Alfredo 
Lundby, Carsten
Pilegaard, Henriette
López Calbet, José Antonio 
UNESCO Clasification: 32 Ciencias médicas
Keywords: Leptin receptors
Leptin signaling
Skeletal muscle
Bed rest
SOCS3, et al
Issue Date: 2014
Publisher: 1439-6319
Journal: European Journal of Applied Physiology 
Abstract: Purpose: This study aimed at determining the effects of bed rest on the skeletal muscle leptin signaling system. Methods: Deltoid and vastus lateralis muscle biopsies and blood samples were obtained from 12 healthy young men (mean ± SD, BMI 22.8 ± 2.7 kg/m2) before and after 7 days of bed rest. Leptin receptor isoforms (OB-Rs), suppressor of cytokine signaling 3 (SOCS3) and protein tyrosine phosphatase 1B (PTP1B) protein expression and signal transducer and activator of transcription 3 (STAT3) phosphorylation were analyzed by Western blot. Results: After bed rest basal insulin concentration was increased by 53 % (P < 0.05), the homeostasis model assessment (HOMA) by 40 % (P < 0.05), and serum leptin concentration by 35 % (P < 0.05) with no changes in body fat mass. Although the soluble isoform of the leptin receptor (s-OBR) remained unchanged, the molar excess of leptin over sOB-R was increased by 1.4-fold after bed rest (P < 0.05). OB-Rs and SOCS3 protein expression, and STAT3 phosphorylation level remained unaffected in deltoid and vastus lateralis by bed rest, as PTP1B in the deltoid. PTP1B was increased by 90 % with bed rest in the vastus lateralis (P < 0.05). There was a linear relationship between the increase in vastus lateralis PTP1B and the increase in both basal insulin concentrations (r = 0.66, P < 0.05) and HOMA (r = 0.68, P < 0.05) with bed rest. Conclusions: One week of bed rest is associated with increased leptin levels without augmenting STAT3 phosphorylation indicating some degree of leptin resistance in skeletal muscle, which can be explained, at least in part, by an elevation of PTP1B protein content in the vastus lateralis muscle.
ISSN: 1439-6319
DOI: 10.1007/s00421-013-2779-4
Source: European Journal Of Applied Physiology [ISSN 1439-6319], v. 114 (2), p. 345-357
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