Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/43846
Title: Immunization with Eimeria ninakohlyakimovae-live attenuated oocysts protect goat kids from clinical coccidiosis
Authors: Ruiz, Antonio
Muñoz, María Carmen 
Molina, José Manuel
Hermosilla, Carlos
Andrada, Marisa 
Lara, Pedro
Bordón, Elisa 
Pérez, Davinia
López, Adassa María 
Matos, Lorena
Guedes, Aránzazu Carmen
Falcón, Soraya 
Falcón, Yaiza
Martín, Sergio 
Taubert, Anja
Keywords: Challenge-Infected Calves
Tenella Oocysts
Bovis Primary
Chickens
Vaccination
Lambs
Irradiation
Efficacy
Immunity
Antigen
Issue Date: 2014
Publisher: 0304-4017
Journal: Veterinary Parasitology 
Abstract: Caprine coccidiosis, affecting mainly young goat kids around the weaning period, is worldwide the most important disease in the goat industry. Control of caprine coccidiosis is increasingly hampered by resistances developed against coccidiostatic drugs leading to an enhanced need for anticoccidial vaccines. In the current study we conducted an oral immunization trial with live attenuated sporulated Eimeria ninakohlyakimovae oocysts. Sporulated E. ninakohlyakimovae oocysts were attenuated by X-irradiation technique. The experimental design included a total of 18 goat kids divided into the following groups: (i) animals immunized with attenuated E. ninakohlyakimovae oocysts at 5 weeks of age and challenged 3 weeks later with non-irradiated homologous oocysts (group 1); (ii) animals infected with non-attenuated E. ninakohlyakimovae oocysts at 5 weeks of age and challenged 3 weeks later with non-attenuated homologous oocysts (group 2); (iii) animals primary-infected with untreated E. ninakohlyakimovae oocysts at 8 weeks of age (control of the challenge infection, group 3); (iv) non-infected control animals (group 4). Goat kids immunized with live attenuated E. ninakohlyakimovae oocysts (group 1) excreted significantly less oocysts in the faeces (95.3% reduction) than kids infected with non-attenuated ones (group 2). Furthermore, immunization with live but attenuated oocysts resulted in ameliorated clinical coccidiosis compared to goat kids infected with untreated oocysts (group 2) and resulted in equally reduced signs of coccidiosis after challenge infection compared to acquired immunity driven by non-attenuated oocysts. Overall, the present study demonstrates for the first time that live attenuated E. ninakohlyakimovae oocysts orally administered showed almost no pathogenicity but enough immunogenicity in terms of immunoprotection. Importantly, vaccinated animal's still shed low amounts of oocysts, guaranteeing environmental contamination and consecutive booster infections to sustain ongoing immunity. (C) 2013 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/10553/43846
ISSN: 0304-4017
DOI: 10.1016/j.vetpar.2013.09.032
Source: Veterinary Parasitology[ISSN 0304-4017],v. 199, p. 8-17
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