Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/43393
Campo DC Valoridioma
dc.contributor.authorPérez, J.en_US
dc.contributor.authorBautista, M. J.en_US
dc.contributor.authorRodríguez Guisado, Franciscoen_US
dc.contributor.authorWilkinson, P. J.en_US
dc.contributor.authorSierra, M. A.en_US
dc.contributor.authorMartín De Las Mulas, J.en_US
dc.date.accessioned2018-11-21T14:48:49Z-
dc.date.available2018-11-21T14:48:49Z-
dc.date.issued1997en_US
dc.identifier.issn0042-4900en_US
dc.identifier.urihttp://hdl.handle.net/10553/43393-
dc.description.abstractBone marrow samples from pigs infected with the highly virulent Malawi'83 or moderately virulent Dominican Republic (DR'78) isolates of African swine fever virus were studied by means of a double labelling immunohistochemical technique which stained the major structural protein VP73 of the virus and megakaryocytes simultaneously. In pigs infected with the highly virulent Malawi'83 isolate, 2·2 per cent of megakaryocytes were vp73+ five days after inoculation, and at six and seven days 2·5 and 9·5 per cent of megakaryocytes were VP73+. Some infected and uninfected megakaryocytes showed pyknosis and karyorrhexis, particularly at seven days after inoculation. However, in comparison with uninfected pigs, the number of megakaryocytes decreased only at seven days after inoculation. In pigs infected with the moderately virulent DR'78 isolate, only 0-2 per cent of megakaryocytes were VP73+ at eight days after inoculation. However, at eight, nine and 10 days after inoculation the total number of megakaryocytes was significantly lower (P<0·01) than in control uninfected pigs, and the majority of the megakaryocytes showed signs of cell death such as pyknosis and karyorrhexis. The fact that this greater destruction of megakaryocytes was associated with the lower rate of infection of this cell type suggests that indirect damage to megakaryocytes is an additional mechanism of thrombocytopenia in acute and subacute African swine fever.en_US
dc.languageengen_US
dc.publisher0042-4900-
dc.relation.ispartofVeterinary Recorden_US
dc.sourceVeterinary Record [ISSN 0042-4900], v. 141, p. 386-390en_US
dc.subject310907 Patologíaen_US
dc.subject.otherImmunohistochemical studyen_US
dc.subject.otherMegakaryocytesen_US
dc.subject.otherAfrican swine feveren_US
dc.titleDouble-labelling immunohistochemical study of megakaryocytes in African swine feveren_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1136/vr.141.15.386en_US
dc.identifier.scopus0031565008-
dc.contributor.authorscopusid55597088351-
dc.contributor.authorscopusid55597088351-
dc.contributor.authorscopusid7006592846-
dc.contributor.authorscopusid21641919200-
dc.contributor.authorscopusid7201434537-
dc.contributor.authorscopusid35516453400-
dc.contributor.authorscopusid57211742924-
dc.contributor.authorscopusid57211742924-
dc.description.lastpage390en_US
dc.description.firstpage386en_US
dc.relation.volume141en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.date.coverdateOctubre 1997en_US
dc.identifier.ulpgces
dc.description.jcr1,081
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUSA-ONEHEALTH1: Epidemiología, Medicina Preventiva Veterinaria y Zoonosis-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.orcid0000-0002-4968-5333-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameRodríguez Guisado, Francisco-
Colección:Artículos
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