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Title: Immunohistochemical detection of Mycoplasma agalactiae in formalin-fixed, paraffin-embedded tissues from naturally and experimentally infected goats
Authors: Rodríguez Guisado, Francisco 
Ramírez, G. A.
Ramírez Corbera, Ana Sofía 
Ball, H. J.
Espinosa de los Monteros y Zayas, Antonio 
Fernández Rodríguez, Antonio Jesús 
UNESCO Clasification: 310907 Patología
320505 Enfermedades infecciosas
Keywords: Goats
Mycoplasma agalactiae
Issue Date: 2002
Publisher: 0931-1793
Journal: Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health 
Abstract: Samples from the mammary tissue of 14 lactating goats (12 naturally infected and two experimentally infected) were examined for the presence of Mycoplasma agalactiae. A monoclonal antibody (5G12) was applied to formalin-fixed, paraffin-wax-embedded sections and labelled by the avidin-biotin peroxidase complex (ABC) method. Histological examination of tissue sections revealed strong immunoreactivity in all animals included in the study. Mycoplasma agalactiae antigen was mainly detected in the cellular debris at the periphery of purulent exudates present within lactiferous sinuses, and lactiferous and interlobular ducts. In addition, M. agalactiae organisms appeared in the cytoplasm of the epithelium of ducts, and in infiltrating macrophages and neutrophils within the ducts, alveoli, interstitial tissue and regional lymph node sinuses. It is concluded that this monoclonal antibody-based immunohistochemical technique is an efficient and specific method for the post-mortem detection of M. agalactiae in cases of clinical mastitis as well as being a useful tool for the study of the route of infection and cellular types involved during mastitis caused by this organism.
ISSN: 0931-1793
DOI: 10.1046/j.1439-0450.2002.00551.x
Source: Journal of Veterinary Medicine, Series B [ISSN 0931-1793], v. 49, p. 226-229
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