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Title: Expression of cell cycle regulators, 14-3-3σ and p53 proteins, and vimentin in canine transitional cell carcinoma of the urinary bladder
Authors: Suarez Bonnet, Alejandro 
Herráez Thomas, Pedro Manuel 
Aguirre Sanceledonio, María 
Suárez-Bonnet, Elena
Andrada Borzollino, Marisa Ana 
Rodríguez Guisado, Francisco 
Espinosa de los Monteros y Zayas, Antonio 
UNESCO Clasification: 310907 Patología
Keywords: 14-3-3σ
Bladder cancer
Transitional cell carcinoma
Issue Date: 2015
Publisher: 1078-1439
Journal: Urologic Oncology: Seminars and Original Investigations 
Abstract: OBJECTIVES: The study of the expression of 14-3-3σ, p53, and vimentin proteins in canine transitional cell carcinoma (TCC) evaluating differences with normal bladder tissues, and the association with clinicopathological variables. METHODS: We analyze by immunohistochemistry in 19 canine TCCs the expression of 14-3-3σ, p53, and vimentin using monoclonal antibodys. A semiquantitative scoring method was employed and statistical analysis was performed to display relationships between variables. RESULTS: In contrast to normal urinary bladder epithelium, which showed high levels of 14-3-3σ, its expression was decreased in 53% of the studied tumors (P = 0.0344). The 14-3-3σ protein was expressed by neoplastic emboli and by highly infiltrative neoplastic cells. The p53 protein was expressed in 26% of TCCs, but no significant association between 14-3-3σ and p53 was detected. Neoplastic epithelial cells displayed vimentin immunoreactivity in 21% of TCCs, and a positive correlation with mitotic index was observed (P = 0.042). Coexpression of vimentin and 14-3-3σ by highly infiltrative neoplastic cells was also observed. CONCLUSIONS: 14-3-3σ is deregulated in canine TCCs and its expression by highly infiltrative tumor cells may be related to the acquisition of aggressive behavior. Furthermore, this article reinforce the role of canine TCC as relevant model of human urothelial carcinoma and we suggest 14-3-3σ as a potential therapeutic target. Further studies are necessary to clarify the role of 14-3-3σ in canine TCC.
ISSN: 1078-1439
DOI: 10.1016/j.urolonc.2015.04.006
Source: Urologic Oncology: Seminars and Original Investigations [ISSN 1078-1439], v. 33(7), p. 332.e1-332.e7
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