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Title: A semi-defined medium without serum for small ruminant mycoplasmas
Authors: Ramírez Corbera, Ana Sofía 
Fleitas, Juan Luis
Rosales Santana, Rubén Sebastián 
Poveda Turrado, Carlos Guillermo 
de la Fe, Christian
Andrada Borzollino, Marisa Ana 
Castro Alonso, Ayoze 
Poveda Guerrero, José Bismarck 
UNESCO Clasification: 310905 Microbiología
310907 Patología
Keywords: Mollicutes
Mycoplasma species
Culture media
Animal sera
Issue Date: 2008
Publisher: 1090-0233
Journal: Veterinary Journal 
Abstract: The composition of the medium used to cultivate Mycoplasma species is very important. Serum is one of the most important additives as it contains lipids (cholesterol) and serum proteins, which are essential for the growth of the organisms. This work reports the development of a semi-defined medium, called MWS (Medium Without Serum) produced without animal serum and bovine serum albumin. MWS seems to be suitable for cultivating several species of caprine mycoplasma, especially M. mycoides subsp. mycoides (LC) and M. mycoides subsp. capri
ISSN: 1090-0233
DOI: 10.1016/j.tvjl.2007.07.023
Source: Veterinary Journal[ISSN 1090-0233],v. 178, p. 149-152
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