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Title: Influence of the dietary inclusion of Gracilaria cornea and Ulva rigida on the biodiversity of the intestinal microbiota of Sparus aurata juveniles
Authors: Rico, R.M.
Tejedor-Junco, M. T. 
Tapia-Paniagua, S.T.
Alarcón, F.J.
Mancera, J.M.
López-Figueroa, F.
Balebona, M.C.
Abdala-Díaz, R.T.
Moriñigo, M.A.
UNESCO Clasification: 2414 Microbiología
251092 Acuicultura marina
Keywords: DGGE
Gilthead sea bream
Gut microbiota
Issue Date: 2016
Journal: Aquaculture International 
Abstract: Partial substitutions of fish meal by 5, 15, or 25 % of Gracilaria cornea or Ulva rigida in experimental diets were evaluated to study their effects on biodiversity of intestinal microbiota composition in gilthead seabream (Sparus aurata) juveniles. The diets were offered to duplicate groups of 15 juvenile fish (14.0 ± 0.5 g) for 70 days, and at the end of the experiment the intestinal microbiota from four specimens of each treatment was analysed by denaturing gel gradient electrophoresis. Results showed that the substitution of fish meal by algae meal induced important modifications in the intestinal microbiota community, as a big reduction of the biodiversity when the highest percentage (25 %) of U. rigida was included. On the contrary, an increase on the number of species was detected when a 15 % of algae was included. Various Lactobacillus delbrueckii subspecies were selectively stimulated when G. cornea was included in the feed, and other bacterial species, such as those included in the Vibrio genus, were reduced.
ISSN: 0967-6120
DOI: 10.1007/s10499-015-9964-x
Source: Aquaculture International [ISSN 0967-6120], v. 24 (4), p. 965-984
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