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Title: Kinship analysis and allelic dropout: a forensic approach on an archaeological case
Authors: Palomo-Díez, Sara
Esparza Arroyo, Ángel
Tirado-Vizcaíno, Mirian
Velasco Vázquez, Javier 
López-Parra, Ana María
Gomes, Cláudia
Baeza-Richer, Carlos
Arroyo-Pardo, Eduardo
UNESCO Clasification: 550501 Arqueología
Keywords: Pcr Amplification Kit
Ancient Dna
Multiplex, et al
Issue Date: 2018
Publisher: 0301-4460
Journal: Annals of Human Biology 
Abstract: Background: This study relies on the discovery of two pit burials (LTA and LTB) of the Bronze Age Cogotas I archaeological culture (circa 3600-2950BP) in Spain. LTA was a single burial and LTB contained three skeletal remains of two adults and a newborn or foetus at term.Aim: The central question posed by this find was whether the LTB tomb constituted a traditional nuclear family (father, mother and son or daughter).Methods: Ancient and forensic DNA protocols were employed to obtain reliable results. Autosomal, X-STR markers and mitochondrial DNA were amplified. Subsequently, different kinship probabilities were estimated by means of LR values calculated using the Familias 3 software. Furthermore, an allelic dropout sensitivity test was developed in order to evaluate the influence of allelic dropout phenomena on the results.Results: It was possible to determine the molecular sex of all individuals and to establish a maternal relationship between the perinatal individual and one of the adults.Conclusion: The remains in the LTB tomb were not a traditional nuclear family (father, mother and son/daughter) and it was probably a tomb where two women, one of them pregnant, were buried.
ISSN: 0301-4460
DOI: 10.1080/03014460.2018.1484159
Source: Annals of Human Biology [ISSN 0301-4460], v. 45 (4), p. 365-368
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