Please use this identifier to cite or link to this item:
Title: Nanobody-mediated resistance to Grapevine fanleaf virus in plants
Authors: Hemmer, Caroline
Djennane, Samia
Ackerer, Léa
Hleibieh, Kamal
Marmonier, Aurélie
Gersch, Sophie
Garcia, Shahinez
Vigne, Emmanuelle
Komar, Véronique
Perrin, Mireille
Gertz, Claude
Belval, Lorène
Berthold, François
Monsion, Baptiste
Schmitt-Keichinger, Corinne
Lemaire, Olivier
Lorber, Bernard
Gutiérrez, Carlos 
Muyldermans, Serge
Demangeat, Gérard
Ritzenthaler, Christophe
UNESCO Clasification: 3109 Ciencias veterinarias
3108 Fitopatología
Keywords: Nanobodies
Plant virus
Transgenic plant
Nepovirus, et al
Issue Date: 2018
Journal: Plant Biotechnology Journal 
Abstract: Since their discovery, single-domain antigen-binding fragments of camelid-derived heavy-chain-only antibodies, also known as nanobodies (Nbs), have proven to be of outstanding interest as therapeutics against human diseases and pathogens including viruses, but their use against phytopathogens remains limited. Many plant viruses including Grapevine fanleaf virus (GFLV), a nematode-transmitted icosahedral virus and causal agent of fanleaf degenerative disease, have worldwide distribution and huge burden on crop yields representing billions of US dollars of losses annually, yet solutions to combat these viruses are often limited or inefficient. Here, we identified a Nb specific to GFLV that confers strong resistance to GFLV upon stable expression in the model plant Nicotiana benthamiana and also in grapevine rootstock, the natural host of the virus. We showed that resistance was effective against a broad range of GFLV isolates independently of the inoculation method including upon nematode transmission but not against its close relative, Arabis mosaic virus. We also demonstrated that virus neutralization occurs at an early step of the virus life cycle, prior to cell-to-cell movement. Our findings will not only be instrumental to confer resistance to GFLV in grapevine, but more generally they pave the way for the generation of novel antiviral strategies in plants based on Nbs.
ISSN: 1467-7644
DOI: 10.1111/pbi.12819
Source: Plant Biotechnology Journal [ISSN 1467-7644], v. 16 (2), p. 660-671
Appears in Collections:Artículos
Adobe PDF (1,24 MB)
Show full item record

Google ScholarTM




Export metadata

Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.