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Title: Three-dimensional cell neighbourhood impacts differentiation in the inner mass cells of the mouse blastocyst
Authors: Fischer, Sabine C.
Corujo-Simon, Elena
Lilao-Garzon, Joaquin
Stelzer, Ernst H. K.
Munoz Descalzo, Silvia 
UNESCO Clasification: 2403 Bioquímica
Keywords: Mouse blastocyst
Primitive endoderm
Image analysis
Three-dimensional analysis
Issue Date: 2017
Abstract: During mammalian blastocyst development, inner cell mass (ICM) cells differentiate into epiblast (Epi) or primitive endoderm (PrE). These two fates are characterised by the transcription factors NANOG and GATA6, respectively. Here, we present quantitative three-dimensional single cell-based neighbourhood analyses to investigate the spatial distribution of NANOG and GATA6 expression in the ICM of the mouse blastocyst. The cell neighbourhood is characterised by the expression levels of the fate markers in the surrounding cells, together with the number of surrounding cells and cell position. We find that cell neighbourhoods are established in early blastocysts and different for cells expressing different levels of NANOG and GATA6. Highest NANOG expressing cells occupy specific positions within the ICM and are surrounded by 9 neighbours, while GATA6 expressing cells cluster according to their GATA6 levels. The analysis of mutants reveals that NANOG local neighbourhood is regulated by GATA6.
DOI: 10.1101/159301
Source: bioRxiv, Jul. 4, 2017; doi:
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