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Title: Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins
Authors: Ponce-González, Jesús G.
Ara, Ignacio
Larsen, Steen
Guerra, Borja 
Calbet, Jose A. L. 
Helge, J. W.
UNESCO Clasification: 32 Ciencias médicas
Keywords: Fat oxidation
Mitochondrial biogenesis
Regional differences and obesity
Issue Date: 2016
Project: Integracion de Los Grupos de la Obesidad y El Síndrome Metabólico .... 
Journal: European Journal of Applied Physiology 
Abstract: Purpose: The aim of this study was to determine if the expression of the mitochondrial biogenesis-regulating proteins SIRT1, SIRT3 and PGC-1alpha in human skeletal muscle is influenced by adiposity. Method: Twenty-nine male subjects were recruited into three groups: control (n = 10), obese (n = 10) and post-obese (n = 9). Intentionally, groups were matched by age, aerobic capacity and in addition the control and post-obese groups also by BMI. Muscle biopsies were obtained from the m. deltoid and vastus lateralis. PGC-1alpha, SIRT1 and SIRT3 protein expression was analyzed by Western blot. Result: PGC-1alpha, SIRT1 and SIRT3 protein expression was similar regardless of the level of adiposity. Only a main effect of group on SIRT1 protein showed a trend toward higher expression in post-obese than control and obese (P = 0.09). Despite similar muscle fiber-type composition (previously reported), PGC-1alpha, SIRT1 and SIRT3 protein expression was higher in leg compared to arm muscle in all groups (P < 0.05). Conclusion: This study shows that PGC-1alpha, SIRT1 and SIRT3 protein expression in basal conditions was not altered in humans with different levels of adiposity but similar aerobic capacity. The expression of PGC-1alpha, SIRT1 and SIRT3 was higher in vastus lateralis than in deltoid muscle, indicating that local rather than systemic factors prevail in regulating the level of expression of these proteins. © 2015, Springer-Verlag Berlin Heidelberg.
ISSN: 1439-6319
DOI: 10.1007/s00421-015-3232-7
Source: European Journal Of Applied Physiology[ISSN 1439-6319],v. 116 (1), p. 11-18
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