Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/41353
Title: Novel methodology to isolate microplastics from vegetal-rich samples
Authors: Herrera, Alicia 
Garrido-Amador, Paloma
Martínez, Ico 
Samper, Maria Dolores
Lopez-Martinez, Juan
Gomez, May 
Packard, Theodore T. 
UNESCO Clasification: 251001 Oceanografía biológica
241705 Biología marina
Keywords: Marine litter
Microplastics
Plastic extraction
Density separation
Organic material
Beach
Issue Date: 2018
Journal: Marine Pollution Bulletin 
Abstract: Microplastics are small plastic particles, globally distributed throughout the oceans. To properly study them, all the methodologies for their sampling, extraction, and measurement should be standardized. For heterogeneous samples containing sediments, animal tissues and zooplankton, several procedures have been described. However, definitive methodologies for samples, rich in algae and plant material, have not yet been developed. The aim of this study was to find the best extraction protocol for vegetal-rich samples by comparing the efficacies of five previously described digestion methods, and a novel density separation method. A protocol using 96% ethanol for density separation was better than the five digestion methods tested, even better than using H2O2 digestion. As it was the most efficient, simple, safe and inexpensive method for isolating microplastics from vegetal rich samples, we recommend it as a standard separation method.
URI: http://hdl.handle.net/10553/41353
ISSN: 0025-326X
DOI: 10.1016/j.marpolbul.2018.02.015
Source: Marine Pollution Bulletin[ISSN 0025-326X],v. 129, p. 61-69
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