Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/37180
DC FieldValueLanguage
dc.contributor.authorSatta, E.en_US
dc.contributor.authorNanni, I. M.en_US
dc.contributor.authorContaldo, N.en_US
dc.contributor.authorCollina, M.en_US
dc.contributor.authorPoveda, José B.en_US
dc.contributor.authorRamírez, Ana S.en_US
dc.contributor.authorBertaccini, A.en_US
dc.date.accessioned2018-05-28T08:44:29Z-
dc.date.available2018-05-28T08:44:29Z-
dc.date.issued2017en_US
dc.identifier.issn0890-8508en_US
dc.identifier.urihttp://hdl.handle.net/10553/37180-
dc.description.abstractPhytoplasmas and mycoplasmas are bacteria belonging to the class Mollicutes. In this study, a fine tuning of quantitative polymerase chain reaction (qPCR) with a universal mycoplasma primer pair (GPO3F/MGSO) targeting the 16S rRNA gene was carried out on phytoplasmas. The dissociation curves of DNAs from Catharanthus roseus phytoplasma-infected micropropagated shoots and from phytoplasma field-infected plant samples showed a single peak at 82.5 °C (±0.5) specifically detecting phytoplasmas belonging to several ribosomal groups. Assay specificity was determined with DNA of selected bacteria: ‘Candidatus Liberibacter solanacearum’, Xylella fastidiosa, Ralstonia solanacearum and Clavibacter michiganensis. No amplification curves were observed with any of these tested bacteria except ‘Ca. L. solanacearum’ that was amplified with a melting temperature at 85 °C. Absolute quantification of phytoplasma titer was calculated using standard curves prepared from serial dilutions of plasmids containing the cloned fragment GPO3F/MGSO from European stone fruit yellows phytoplasma. Phytoplasma copy number ranged from 106 to 103 according with the sample. The sensitivity evaluated comparing plasmid serial dilutions resulted 10−6 for conventional PCR and 10−7 for qPCR. The latter method resulted therefore able to detect very low concentrations of phytoplasma in plant material.en_US
dc.languageengen_US
dc.relation.ispartofMolecular and Cellular Probesen_US
dc.sourceMolecular and Cellular Probes[ISSN 0890-8508],v. 35, p. 1-7en_US
dc.subject32 Ciencias médicasen_US
dc.subject.otherMollicutesen_US
dc.subject.otherProkaryotesen_US
dc.subject.otherDiagnosticen_US
dc.subject.otherPlant diseasesen_US
dc.subject.otherQuantificationen_US
dc.titleGeneral phytoplasma detection by a q-PCR method using mycoplasma primersen_US
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1016/j.mcp.2017.05.008
dc.identifier.scopus85019716167
dc.identifier.isi000412793600001-
dc.contributor.authorscopusid57189579314
dc.contributor.authorscopusid56418230400
dc.contributor.authorscopusid35279369600
dc.contributor.authorscopusid24334025600
dc.contributor.authorscopusid7005736558
dc.contributor.authorscopusid7401735183
dc.contributor.authorscopusid7005650463
dc.description.lastpage7-
dc.description.firstpage1-
dc.relation.volume35-
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.date.coverdateOctubre 2017
dc.identifier.ulpgces
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptEpidemiología y Medicina Preventiva Veterinaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptEpidemiología y Medicina Preventiva Veterinaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-9846-2427-
crisitem.author.orcid0000-0002-8721-775X-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNamePoveda Guerrero, José Bismarck-
crisitem.author.fullNameRamírez Corbera, Ana Sofía-
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