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Title: Determination of steroid hormones in fish tissues by microwave-assisted extraction coupled to ultra-high performance liquid chromatography tandem mass spectrometry
Authors: Guedes-Alonso, Rayco 
Sosa-Ferrera, Zoraida 
Santana-Rodríguez, José Juan 
UNESCO Clasification: 2391 Química ambiental
2403 Bioquímica
Keywords: Fish tissue
Mass spectrometry
Microwave-assisted extraction
Solid phase extraction
Steroid hormones
Ultra-high-performance liquid
Issue Date: 2017
Journal: Food Chemistry 
Abstract: Steroid hormones produce adverse effects on biota as well as bioaccumulation in fish and seafood, making it necessary to develop methodologies to evaluate these compounds in samples related to the food chain. This work presents an analytical method for evaluating 15 steroid hormones in fish tissue. It is based on microwave-assisted extraction and solid-phase extraction coupled to ultra-high-performance liquid chromatography tandem mass spectrometry (MAE-SPE-UHPLC-MS/MS). The proposed method shows appropriate detection limits (0.14–49.0 ng g−1), recoveries in the range of 50% and good repeatability. After optimization, the method was applied to different tissues from two small fishes of the Canary Islands that constitute an important level of the food web (Boops boops and Sphoeroides marmoratus) and were exposed to the outfall of the Las Palmas de Gran Canaria wastewater treatment plant. The concentrations of eight detected compounds ranged from below the quantification limits to 3.95 μg g−1.
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2017.06.065
Source: Food Chemistry[ISSN 0308-8146],v. 237, p. 1012-1020
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