Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/35685
DC FieldValueLanguage
dc.contributor.authorMatos, L.en_US
dc.contributor.authorMuñoz, M. C.en_US
dc.contributor.authorMolina Caballero, José Manuelen_US
dc.contributor.authorRodríguez, F.en_US
dc.contributor.authorPérez Barreto, Daviniaen_US
dc.contributor.authorLopez, A.en_US
dc.contributor.authorFerrer, O.en_US
dc.contributor.authorHermosilla, C.en_US
dc.contributor.authorTaubert, A.en_US
dc.contributor.authorRuiz Reyes, Antonioen_US
dc.date.accessioned2018-04-26T07:57:32Z-
dc.date.available2018-04-26T07:57:32Z-
dc.date.issued2017en_US
dc.identifier.issn0304-4017en_US
dc.identifier.urihttp://hdl.handle.net/10553/35685-
dc.description.abstractDuring the first schizogony, the goat coccidia Eimeria ninakohlyakimovae develops macroschizonts in lacteal duct endothelial cells, whose rupture leads to severe ileal damage and clinical signs during the prepatent period. The immune response elicited against early stages of the parasite development still requires to be investigated. In the present study we have evaluated immune reactions in goat kids primary- and challenged-infected with Eimeria ninakohlyakimovae, and sacrificed during prepatency (7 days after challenge). The oocyst output during the primary infection, body weight and clinical condition of all the animals were examined and, at the end of the experiment, all the goat kids were euthanized and subjected to necropsy. Samples were taken from different sections of the ileum, colon and mesenteric lymph nodes (MLN) of primary- and challenged E. ninakohlyakimovae-infected animals. Intestinal leukocyte subpopulations were characterized in E. ninakohlyakimovae-infected mucosa and counts of lymphocytes, eosinophils, polymorphonuclear neutrophils (PMN), globular leukocytes and mast cells were recorded. Additionally, gene expression of caprine IL-2, IL-4, IL-10 and INF gamma of ileal, colonic and MLN tissues were performed, as well as the immunohistochemical characterization of immune cells. The E. ninakohlyakimovae primary infection resulted in moderate to severe enteritis with different degrees of diarrhoea and was accompanied by high OPG counts and an increase of most immune cells analyzed when compared to uninfected control animals. Furthermore, eosinophil-, lymphocyte-, globular leukocyte- and mast cell-counts were significantly higher in the challenge group compared to the primary infected animals, whilst the opposite was true for PMN counts. The challenge infection was also associated with moderate increased levels of local mucosal IgA. Interestingly, the number of immature schizonts found at the ileal mucosa was statistically higher in the challenge infected group compared to the challenged control animals. Furthermore, in the challenged E. ninakohlyakimovae-infected animals a significantly higher number of mucosal CD4(+) and CD8(+) lymphocytes were observed, indicating that these T cell subpopulations might be involved in protective host immune response elicited against early stages of parasite development. The immune response was however very complex, as antigen presenting cells and other effector cell populations of the innate immune system, as well as certain cytokines, were involved. In summary, the results of this study contribute to the better understanding of local cellular and humoral immune responses against caprine E. ninakohlyakimovae, particularly during the prepatency.en_US
dc.languageengen_US
dc.relation.ispartofVeterinary Parasitologyen_US
dc.sourceVeterinary Parasitology[ISSN 0304-4017],v. 242, p. 1-9en_US
dc.subject3109 Ciencias veterinariasen_US
dc.subject.otherEimeria ninakohlyakimovaeen_US
dc.subject.otherGoatsen_US
dc.subject.otherPrepatencyen_US
dc.subject.otherImmune responseen_US
dc.titleProtective immune responses during prepatency in goat kids experimentally infected with Eimeria ninakohlyakimovaeen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1016/j.vetpar.2017.04.016
dc.identifier.scopus85019915747
dc.identifier.isi000404697600001-
dc.contributor.authorscopusid37026514900
dc.contributor.authorscopusid57199669332
dc.contributor.authorscopusid55731962600
dc.contributor.authorscopusid21641919200
dc.contributor.authorscopusid36096860400
dc.contributor.authorscopusid54784854300
dc.contributor.authorscopusid7003583407
dc.contributor.authorscopusid6602143835
dc.contributor.authorscopusid6603706756
dc.contributor.authorscopusid57201387360
dc.identifier.eissn1873-2550-
dc.description.lastpage9-
dc.description.firstpage1-
dc.relation.volume242-
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid3230112
dc.contributor.daisngid29395753
dc.contributor.daisngid8060323
dc.contributor.daisngid2263409
dc.contributor.daisngid3415357
dc.contributor.daisngid5350027
dc.contributor.daisngid28182072
dc.contributor.daisngid319992
dc.contributor.daisngid384245
dc.contributor.daisngid1497623
dc.contributor.wosstandardWOS:Matos, L
dc.contributor.wosstandardWOS:Munoz, MC
dc.contributor.wosstandardWOS:Molin, JM
dc.contributor.wosstandardWOS:Rodriguez, F
dc.contributor.wosstandardWOS:Perez, D
dc.contributor.wosstandardWOS:Lopez, A
dc.contributor.wosstandardWOS:Ferrer, O
dc.contributor.wosstandardWOS:Hermosilla, C
dc.contributor.wosstandardWOS:Taubert, A
dc.contributor.wosstandardWOS:Ruiz, A
dc.date.coverdateAgosto 2017
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.deptParasitología, dermatologia y biopatologia veterinaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.deptParasitología, dermatologia y biopatologia veterinaria-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.deptEpidemiología y Medicina Preventiva Veterinaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.deptParasitología, dermatologia y biopatologia veterinaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.deptParasitología, dermatologia y biopatologia veterinaria-
crisitem.author.orcid0000-0002-4968-5333-
crisitem.author.orcid0000-0001-9038-042X-
crisitem.author.orcid0000-0003-0668-5496-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.fullNameMuñoz Ojeda, María Del Carmen-
crisitem.author.fullNameMolina Caballero, José Manuel-
crisitem.author.fullNameRodríguez Guisado, Francisco-
crisitem.author.fullNameLópez González, Adassa María-
crisitem.author.fullNameFerrer Quintana, Otilia Rosa-
crisitem.author.fullNameRuiz Reyes, Antonio-
crisitem.author.departamentoPatología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.departamentoPatología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.departamentoMorfología-
crisitem.author.departamentoPatología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.departamentoPatología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.departamentoPatología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
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