Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/35329
Title: Sex molecular diagnosis on critical samples: Comparison of different methodologies
Authors: Palomo-Díez, Sara
Gomes, Cláudia
Martínez-Labarga, Cristina
Lelli, Roberta
Rickards, Olga
Velasco Vázquez, Javier 
Esparza-Arroyo, Ángel
Arroyo-Pardo, Eduardo
UNESCO Clasification: 2402 Antropología (física)
550405 Prehistoria
Keywords: Low Template DNA (LTD)
Autosomal STR
Amelogenin
X-Indels
Issue Date: 2017
Journal: Forensic Science International: Genetics Supplement Series 
Abstract: A fundamental question of the personal identification is the sex determination. Sometimes, the anthropological sex diagnosis is not trustworthy; especially when facing up to sub-adult skeletal remains or very degraded samples. Because of that, the molecular analysis could constitute a fundamental tool to establish a reliable sex diagnosis. Nevertheless, when analysing critical samples, the molecular sex diagnosis could show some difficulties when dealing with Low Template DNA (LTD) and stochastic phenomena. Through this study 4 different methodologies were compared for the molecular sex diagnosis on critical samples (LTD samples). Concretely, 60 samples were analysed from ancient human skeletal remains of different antiquity (Chalcolithic and Bronze Age). The sex diagnose was determined by 2 different autosomal STR commercial kits (AmpFℓSTR® MiniFiler™ PCR Amplification Kit, and AmpFLSTR® NGM™ PCR Amplification Kit), which includes an amelogenine gen marker; The other methodology contemplated the amelogenin gen amplification also, performed by real time PCR (RTPCR). The last technique was based on the Insertion-Deletion Polymorphisms of the X-chromosome analysis (X-INDELs). Through these analyses, we compare the 4 different methods, evaluating advantages and disadvantages and comparing also with the anthropological sex diagnosis
URI: http://hdl.handle.net/10553/35329
ISSN: 1875-1768
DOI: 10.1016/j.fsigss.2017.09.168
Source: Forensic Science International: Genetics Supplement Series [ISSN 1875-1768], Vol. 6, p. e385-e387
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