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Title: Genetic characterization and determination of the number of individuals by molecular analysis in a prehistoric finding
Authors: Palomo-Díez, Sara
Esparza-Arroyo, Ángel
Gomes, Cláudia
Velasco Vázquez, Javier 
López-Parra, Ana María 
Baeza Richer, Carlos
Martínez-Labarga, Cristina
Arroyo-Pardo, Eduardo
Rickards, Olga 
UNESCO Clasification: 2402 Antropología (física)
550405 Prehistoria
550501 Arqueología
Keywords: Ancient DNA
Iberian Peninsule
Kinship analysis
STR analysis, et al
Issue Date: 2017
Journal: Forensic Science International: Genetics Supplement Series 
Abstract: The present study focuses on the genetic analysis of skeletal human remains exhumed from a ritual burial located in Los Cercados Chalcolithic site (3970 ± 60 BP) (Valladolid, Central Spain). In this burial different pottery and animal remains were found, configuring a complex ritual, accompanied by scarce human remains, concretely a maxilla and three skulls without maxilla. The most striking aspect of these human remains was the different impact trauma signs on the back side of the skulls. The anthropological analysis established that the skulls were typical feminine, The bad state of preservation of the maxilla did not allowed to assign this to any of the three skulls. So, it was not possible to determine the number of individuals by anthropological methodology. However, we could determine the number of individuals by the genetic analysis of autosomal STRs and mitochondrial DNA on the skeletal remains. It was possible to assign the maxilla to one of other three human skulls. On the other hand, we have been able to verify the sex of each individual by molecular analysis. Finally, a kinship analysis among the individuals was performed using a specific software (Familias 3.0), resulting in a possible sibling relationship between two of the individuals.
ISSN: 1875-1768
DOI: 10.1016/j.fsigss.2017.09.187
Source: Forensic Science International: Genetics Supplement Series [ISSN 1875-1768], v. 6, p. e487-e489
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