Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/259
Title: The effects of the physical characteristics of the culture medium on the development of red seaweeds in tissue culture
Authors: Robaina Romero, Rafael 
García Reina, Guillermo
Luque-Escalona, Angel 
UNESCO Clasification: 241707 Algología (ficología)
Keywords: Algas rojas
Callus
Gelidium versicolor
Grateloupia doryphora
Laurencia sp.
Osmolality
Regeneration
Issue Date: 1990
Journal: Hydrobiologia 
Abstract: Explants of Gelidium versicolor, Grateloupia doryphora and Laurencia sp. were cultivated in Provasoli enriched seawater culture medium (PES) adjusted to several osmolalities (0.5, 0.7, 1.0 and 1.5 Os kg−1) and solidities (agar concentration = 3, 8 and 15 g L−1). Osmolality was adjusted by dilution of seawater with distilled water (50, 70 and 100% seawater) and by NaCl addition. Explants of Laurencia sp. and Grateloupia doryphora showed bud regeneration and callus formation. Explants of Gelidium versicolor only showed bud regeneration. Osmolalities of 0.5 and 1.05 Os kg−1. inhibited or drastically reduced bud regeneration and callus formation. The highest callus formation and bud regeneration were observed at 0.7 to 1.0 Os kg−1. An increase in the agar concentration of the culture medium was positively correlated with callus formation and negatively correlated with bud regeneration. An increase in the percentage of seawater increased the solidity of the culture medium and was positively correlated with callus formation. Glycerol was an effective carbon source for the vegetative propagation of axenic explants of Grateloupia doryphora, promoting growth and bud regeneration. An increase in glycerol concentration in the culture medium increased its osmolality, inhibiting the growth of the explants and their morphogenetic development.
URI: http://hdl.handle.net/10553/259
ISSN: 0018-8158
Source: Hydrobiologia [ISSN 0018-8158], v. 204/205 (1), p. 137-142
Appears in Collections:Artículos
Thumbnail
Adobe PDF (374,13 kB)
Show full item record

Page view(s)

7
checked on Apr 4, 2020

Download(s)

13
checked on Apr 4, 2020

Google ScholarTM

Check


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.